ProHealth fibromyalgia Vitamin and Natural Supplement Store and Health
Log In  |  My Account  |  View Cart  View Your ProHealth Vitamin and Supplement Shopping Cart
800-366-6056  |  Contact Us  |  Help

|
|
|
 
Print Page
Email Article

Cytotoxic lymphocyte microRNAs as prospective biomarkers for Chronic Fatigue Syndrome/Myalgic Encephalomyelitis

  [ 1 vote ]   [ Post a Comment ]
By Ekua W Benu, Sonya M Marshall-Gradisnik, et al. • www.ProHealth.com • October 9, 2012

previous article next article

[Note: microRNAs (miRNAs) are biological regulators of cell function [see video]. Aberrant expression of microRNAs has been implicated in numerous disease states, with the result that investigations to identify, understand and potentially manipulate them represent a cutting-edge field of research.]

Background: Immune dysfunction associated with a disease often has a molecular basis.

A novel group of molecules known as microRNAs (miRNAs) have been associated with suppression of translational processes involved in cellular development and proliferation, protein secretion, apoptosis, immune function and inflammatory processes.

MicroRNAs may be implicated in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME), where immune function is impaired.

The objective of this study was to determine the association between miRNAs in cytotoxic cells and CFS/ME. ['Cytotoxic' denotes 'cell-killing'.]

Methods:

Natural Killer (NK) and CD8(+)T cells were preferentially isolated from peripheral blood mononuclear cells from all participants (CFS/ME, n = 28; mean age = 41.8 +/- 9.6 years; and controls, n = 28; mean age = 45.3 +/- 11.7 years), via negative cell enrichment.

Following total RNA extraction and subsequent synthesis of cDNA, reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) was used to determine the expression levels of nineteen miRNAs.

Results:

• There was a significant reduction in the expression levels of miR-21, in both the NK and CD8(+)T cells in the CFS/ME sufferers.

• Additionally, the expression of miR-17-5p, miR-10a, miR-103, miR-152, miR-146a, miR-106, miR-223 and miR-191 was significantly decreased in NK cells of CFS/ME patients in comparison to the non-fatigued controls.

Limitations: The results from these investigations are not yet transferable into the clinical setting, further validatory studies are now required.

Conclusions:

• Collectively these miRNAs have been associated with apoptosis, cell cycle, development and immune function.

• Changes in miRNAs in cytotoxic cells may reduce the functional capacity of these cells and disrupt effective cytotoxic activity along with other immune functions in CFS/ME patients.

Source: Journal of Affective Disorders, May 7, 2012 [fee-based article]. PMID:22572093, by Brenu EW, Ashton KJ, van Driel M, Staines DR, Peterson D, Atkinson GM, Marshall-Gradisnik SM. Faculty of Health Science and Medicine, Population Health and Neuroimmunology Unit, Bond University, Robina, Queensland, Australia. [Email: ekbrenu@bond.edu.au]



previous article
  Rating 5 (1 votes) next article




DISCUSS THIS ARTICLE   (0 existing comments) Post a Comment 




[ Be the first to comment on this article ]




 
Free Chronic Fatigue Syndrome and Fibromyalgia Newsletters
Subscribe to
Our FREE
Newsletter
Subscribe Now!
Receive up-to-date ME/CFS & Fibromyalgia treatment and research news
 Privacy Guaranteed  |  View Archives

Save on Your Next Order

Featured Products
Guaifenesin FA™ Guaifenesin FA™
Natural Expectorant Relieves Chest Congestion
Fibro Energy Formula Fibro Energy Formula
13 Top Nutrients for Effective Energy Support
Curcumin, Longvida™ by Nutrivene Curcumin, Longvida™ by Nutrivene
Helps Support Cognition, Memory & Overall Health
Vitamin D3 5000 IU Vitamin D3 5000 IU
Give your body a boost with the sunshine vitamin
Fibro Freedom™ Fibro Freedom™
Soothes, strengthens & revitalizes

Most Viewed Articles
What's the Hidden Cause Behind YOUR Fibromyalgia Flares? [more]

Pridgen Reports Fibromyalgia Antiviral Trial Results “Very Positive”: Predicts New Approach Will Be ... [more]

Massage Therapy Studies for Fibromyalgia Reviewed [more]

How to Limit or Minimize Your Fibromyalgia Flare [more]

Mitochondrial Dysfunction, Post-Exertional Malaise and CFS/ME [more]

The Devil Is In The Details – A Herpes Simplex Virus Inquiry For Fibromyalgia and Chronic Fatigue Sy... [more]

Sympathetic Nervous System Dysfunction in FIbromyalgia and Overlapping Conditions [more]

VIDEO: IACFS/ME Conference - Summary of Research by Dr. Komaroff [more]

Review of Nutritional Supplements Used for ME/CFS and FM [more]

FREE: Stop Feeding Yourself PAIN Guide [more]


FIBROMYALGIA RESOURCES
What is Fibromyalgia?
Fibromyalgia 101
Fibromyalgia Symptoms
Fibromyalgia Treatments
| CFS RESOURCES
What is CFS?
ME/CFS 101
ME/CFS Symptoms
ME/CFS Treatments
| FORUMS
Fibromyalgia
ME/CFS
ADVANCED MEDICAL LABS
WHOLESALE  |  AFFILIATES
GUARANTEE
CONTACT US
PRIVACY
RSS
SITE MAP
ProHealth on Facebook  ProHealth on Twitter  ProHealth on Pinterest  ProHealth on Google Plus
Credit Card Processing