ProHealth fibromyalgia Vitamin and Natural Supplement Store and Health
Home  |  Log In  |  My Account  |  View Cart  View Your ProHealth Vitamin and Supplement Shopping Cart
800-366-6056  |  Contact Us  |  Help
Facebook Google Plus
Fibromyalgia  Chronic Fatigue Syndrome & M.E.  Lyme Disease  Natural Wellness  Supplement News  Forums  Our Story
Store     Brands   |   A-Z Index   |   Best Sellers   |   New Products   |   Deals & Specials   |   Under $10   |   SmartSavings Club

Trending News

An Easy Way to Tell if You Have Small-Fiber Neuropathy? The Fibromyalgia Symptom Study

Twenty-Six Years of Hell: An ME/CFS/FM Recovery Story

How to manage a relationship when you have a chronic illness

In Their Own Words: Chronic Fatigue Syndrome and Fibromyalgia Patients Describe Their Symptoms – Par...

The Opioid Painkiller Clampdown – Will it Lead to Better Drugs?

The Dos and Don’ts of Fibromyalgia

An Alternative to Goals and Resolutions: Intentions and Extreme Permissiveness

That was Then…This is Now Life with Fibromyalgia

Top 10 Gifts to People Living with Pain

Relaxation: Part 2 of the multi-treatment approach to pain

 
Print Page
Email Article

Investigation of mast cell toll-like receptor 3 in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis and Systemic Mastocytosis participants using the novel application of autoMACS magnetic separation and flow cytometry

  [ Not Yet Rated ]   [ 1 Comment ]
By C. Balinas et al. • www.ProHealth.com • December 17, 2017


By C. Balinas et al.

Abstract

BACKGROUND: Viral infections and hypersensitivities are commonly reported by Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME) patients. Mast Cells (MC) uniquely mediate type 1 hypersensitivities and resolve viral infections via toll-like receptor 3 (TLR3).

OBJECTIVE: To characterise and compare mast cell progenitors (MCPs) in CFS/ME participants with a known MC disorder, Systemic mastocytosis (SM), and secondly, to investigate the role of MC TLR3 in CFS/ME participants following Polyinosinic:polycytidylic acid (Poly I:C) stimulation.

METHODS: A total of 11 International Consensus Criteria defined CFS/ME participants (40.42 ± 10.31), 9 World Health Organisation defined systemic mastocytosis (SM) participants (47.00 ± 10.37) and 12 healthy controls (HC) (36.36 ± 9.88) were included. Following autoMACS magnetic separation, CD117+/Lin-MCPs were stimulated with Poly I:C for 24hr. MCP purity (CD117 and Lin2), maturity (CD34 and Fc?RI), interaction receptors and ligands (CD154 and HLA-DR), and SM-specific (CD2 and CD25) markers were measured using flow cytometry.

RESULTS: There was a significant decrease in HLA-DR+/CD154- expression between CFS/ME and SM groups pre and post Poly I:C stimulation. There were no significant differences in maturity MCPs, CD154, and CD2/CD25 expression between groups pre and post Poly I:C stimulation.

CONCLUSION: This pilot investigation provides a novel methodology to characterise MCPs in a rapid, inexpensive and less invasive fashion. We report a significant decrease in HLA-DR+/CD154- expression between CFS/ME and SM participants, and an observed increase in HLA-DR-/CD154+ expression post Poly I:C stimulation in CFS/ME participants. Peripheral MCPs may be present in CFS/ME pathophysiology, however further investigation is required to determine their immunological role.

Source: Balinas C, Nguyen T, Johnston S, Smith P, Staines D, Marshall-Gradisnik S. Investigation of mast cell toll-like receptor 3 in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis and Systemic Mastocytosis participants using the novel application of autoMACS magnetic separation and flow cytometry. Asian Pac J Allergy Immunol. 2017 Dec 10. doi: 10.12932/AP-200517-0086. [Epub ahead of print]




Post a Comment

Featured Products From the ProHealth Store
Fibro Freedom™ B-12 Extreme™ Hydroxocobalamin Extreme™


Article Comments Post a Comment

Peripheral MCPs
Posted by: eufaula
Dec 17, 2017
So what exactly are Peripheral MCPs? Please explain so a theater major, NOT a science major, can understand. Thank you!
Reply Reply
 
Optimized Curcumin Longvida with Omega-3

Featured Products

Guaifenesin FA™ Guaifenesin FA™
Helps the Body Eliminate Excess Calcium and Phosphates
Hydroxocobalamin Extreme™ Hydroxocobalamin Extreme™
The B-12 Your Brain Needs for Detox & Sharpness
Fibro Freedom™ Fibro Freedom™
Soothes, Strengthens & Revitalizes
Optimized Curcumin Longvida® Optimized Curcumin Longvida®
Supports Cognition, Memory & Overall Health
Energy NADH™ 12.5mg Energy NADH™ 12.5mg
Improve Energy & Cognitive Function

Natural Remedies

Thyroid Health and Fibromyalgia Thyroid Health and Fibromyalgia
Why Berries Offer a Rainbow of Health Benefits Why Berries Offer a Rainbow of Health Benefits
Natural Bladder Control, Go Less and Live More Natural Bladder Control, Go Less and Live More
How to Jump-start and Sustain Energy Production in CFS How to Jump-start and Sustain Energy Production in CFS
Stop Bacteria With Nature's Antibiotics Stop Bacteria With Nature's Antibiotics

CONTACT US
ProHealth, Inc.
555 Maple Ave
Carpinteria, CA 93013
(800) 366-6056  |  Email

· Become a Wholesaler
· Vendor Inquiries
· Affiliate Program
SHOP WITH CONFIDENCE
Credit Card Processing
SUBSCRIBE TO OUR NEWSLETTERS
Get the latest news about Fibromyalgia, M.E/Chronic Fatigue Syndrome, Lyme Disease and Natural Wellness

CONNECT WITH US ProHealth on Facebook  ProHealth on Twitter  ProHealth on Pinterest  ProHealth on Google Plus

© 2018 ProHealth, Inc. All rights reserved. Pain Tracker App  |  Store  |  Customer Service  |  Guarantee  |  Privacy  |  Contact Us  |  Library  |  RSS  |  Site Map