ProHealth health Vitamin and Natural Supplement Store and Health
Home  |  Log In  |  My Account  |  View Cart  View Your ProHealth Vitamin and Supplement Shopping Cart
800-366-6056  |  Contact Us  |  Help

|
|
|
|

Trending News

Mitochondria-Booster NIAGEN® Shows Promise in First Human Clinical Trial

Exploring the association between Morgellons disease and Lyme disease: identification of Borrelia bu...

New Envita Study Shows Chronic Lyme Disease "Fools" Immune System, Resistant to Antibiotics

Omega-3 fatty acids boost B vitamin brain benefit

Substantial health threat: Never-before-seen tick-borne disease

Trigger identified that likely unleashes autoimmune disease

New records of the Lyme disease bacterium in ticks collected from songbirds in central and eastern C...

Lyme Advocates Call on IDSA to Focus on Patient Care Instead of Attacking Those in Distress

Zinc deficiency linked to chronic inflammation

New Johns Hopkins Lyme Disease Clinical Research Center launched

 
Print Page
Email Article

Rapid Methods for Identification of Poliovirus Isolates and Determination of Polio Neutralizing Antibody Titers in Human Sera

  [ 40 votes ]   [ Discuss This Article ]
www.ProHealth.com • March 11, 2002




J Virol Methods 2002 Mar;101(1-2):189-96

Di Lonardo A, Buttinelli G, Amato C, Novello F, Ridolfi B, Fiore L.

Laboratory of Virology, Istituto Superiore di Sanita', Viale Regina Elena 299, 00161, Rome, Italy

A new rapid method for identification and determination of the titer of polioviruses and other enteroviruses in cell monolayers grown in microtiter plates is described. The method is based on immunoperoxidase staining of infected cells with commercial monoclonal antibodies (MAb) and biotin-labeled secondary antibody.

The presence of poliovirus or other enteroviruses was established as the appearance of at least one focus of cells with stained cytoplasm 6 h post-infection. Viral titers determined by this method were expressed as focus forming unit (FFU) per ml which was found to correspond approximately to 10 TCID(50)/ml. The suitability of this technique to determine poliovirus antibody titers in human sera was also tested comparing the immunocytochemical neutralization assay with a conventional neutralization in microtiter plates.

The test was standardized using reference human sera in order to produce antibody titers expressed in international units (IU). In addition to high reproducibility, the new neutralization test appears to be sensitive, specific and rapid, and might thus represent a useful tool for the diagnosis of polio and other enterovirus infections.

PMID: 11849697 [PubMed - in process]


Please Discuss This Article:   Post a Comment 



[ Be the first to comment on this article ]




 
Free Chronic Fatigue Syndrome and Fibromyalgia Newsletters
Subscribe to
Our FREE
Newsletter
Subscribe Now!
Receive up-to-date ME/CFS & Fibromyalgia treatment and research news
 Privacy Guaranteed  |  View Archives

Save on Nutritional Supplement Orders

Featured Products

Vitamin D3 Extreme™ by ProHealth Vitamin D3 Extreme™ by ProHealth
50,000 IU Vitamin D3 - Prescription Strength
Ultra ATP+, Double Strength Ultra ATP+, Double Strength
Get energized with malic acid & magnesium
Ultra EPA  - Fish Oil Ultra EPA - Fish Oil
Ultra concentrated source of essential fish oils
FibroSleep™ by ProHealth FibroSleep™ by ProHealth
The All-in-One Natural Sleep Aid
Energy NADH™ 12.5mg Energy NADH™ 12.5mg
Improve Energy & Cognitive Function

Natural Remedies

Live Without Anxiety or Stress
Rejuvenating the Brain - How PQQ Helps Power Up Mental Processing Rejuvenating the Brain - How PQQ Helps Power Up Mental Processing
Three-Step Strategy to Reverse Mitochondrial Aging Three-Step Strategy to Reverse Mitochondrial Aging
Eating Fat is Good… Maybe… Could Be… Sometimes Eating Fat is Good… Maybe… Could Be… Sometimes
Coping When Colds or Flu Catch Up with You

FIBROMYALGIA RESOURCES
What is Fibromyalgia?
Fibromyalgia Diagnosis
Fibromyalgia Symptoms
Fibromyalgia Treatments
| CFS RESOURCES
What is CFS?
ME/CFS Diagnosis
ME/CFS Symptoms
ME/CFS Treatments
| FORUMS
Fibromyalgia
ME/CFS
ADVANCED MEDICAL LABS
WHOLESALE  |  AFFILIATES
GUARANTEE  |  PRIVACY
CONTACT US
LIBRARY
RSS
SITE MAP
ProHealth on Facebook  ProHealth on Twitter  ProHealth on Pinterest  ProHealth on Google Plus
Credit Card Processing