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Test of immune activity in ME/CFS indicates it's a condition associated with overactive Th2 response

  [ 26 votes ]   [ Discuss This Article ] • February 16, 2012

Self-test monitoring of the Th1/Th2 balance in health and disease with special emphasis on chronic fatigue syndrome/myalgic encephalomyelitis
– Source: Journal of Medical Laboratory and Diagnosis, Feb 2012
By Chris Roelant and Kenny De Meirleir

[Note: Th (T helper) cells trigger and direct the immune response. Th1 and Th2 are different subsets that muster response to different challenges. Broadly, Th1 musters cellular immunity, and Th2 is involved in humoral immunity (noncellular components of blood such as plasma & lymph) and mucosal immunity and allergy. Different diseases may dysregulate the Th1-Th2 balance: be either Th1 or Th2 mediated/dominant. This quick urine test for Th1/Th2 activity allows evaluation by resulting color of whether a treatment strategy such as antioxidants, nutraceuticals or drug is working to restore balance. And testing of more than 450 chronic fatigue syndrome/ME patients suggests ME/CFS involves heightened Th2 activity.]

To read the full text of this article free, CLICK HERE.
Abstract: A simple “self-test” principle is described which allows patients to evaluate their Th1/Th2 balance repeatedly over short periods of time to follow-up the effects of taking pre- and probiotics, neutraceuticals, drugs or any other therapeutic strategy to balance Th1/Th2 status.

By analyzing a large number of first morning urine samples obtained from individuals with medical conditions associated with an overactive Th2 arm (ulcerative colitis, autism, blastocystis, mercury poisoning and viral infection), a reaction principle was discovered that uses a redox-active colorimetric substrate changing color upon reaction with metabolites present at high concentration in the urine samples of Th2-shifted individuals.

The development of color is time-dependent and quantitative.

Moreover, 75% of urine samples obtained from chronic fatigue/myalgic encephalomyelitis patients produced a time-dependent and quantitative change of color compared to only 4% of the controls (perfectly healthy population), providing evidence that chronic fatigue syndrome/myalgic encephalomyelitis is a condition associated with an overactive Th2 arm.

Source: Journal of Medical Laboratory and Diagnosis, Feb 2012. By Roelant C, De Meirleir K. Protea Biopharma N.V., Neder-Over-Heembeek, Belgium; Department of Human Physiology, Free University of Brussels (VUB), Brussels, Belgium. [Email:

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