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A single recombinant plasmid expressing two major outer surface proteins of the Lyme disease spirochete.

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By Howe TR, Mayer LW, Barbour AG • www.ProHealth.com • February 8, 1985


Abstract

A gene bank of DNA from the Lyme disease spirochete was constructed in the plasmid pBR322. Plasmid pTRH32, a recombinant that in Escherichia coli expresses the two major outer surface proteins of the Lyme disease spirochete, was identified. One of the recombinant products, designated OspA, represents a surface protein that appears to be common to all Lyme disease spirochetes, whereas the other recombinant product, designated OspB, represents a more variable surface protein. This recombinant plasmid provides a foundation for future studies on the epidemiology and pathogenesis of Lyme disease as well as on the genetic organization of the etiologic agent.

Science. 1985 Feb 8;227(4687):645-6.





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