ProHealth health Vitamin and Natural Supplement Store and Health
Home  |  Log In  |  My Account  |  View Cart  View Your ProHealth Vitamin and Supplement Shopping Cart
800-366-6056  |  Contact Us  |  Help

|
|
|
|

Trending News

Strengthening DNA to Prevent Inflammation, Heart Disease, Dementia and More

Optimal Prostate Defense Requires a Multi-Modal Strategy

The Most Powerful Natural Antioxidant Discovered to Date - Hydroxytyrosol

How Lipoic Acid Preserves Critical Mitochondrial Function

Omega-3 Fatty Acids Increase Brain Volume

Magnesium: The Missing Key to Your Health?

New Option For Chronic Fatigue Syndrome

Living Longer, Healthier Lives with Resveratrol

Iodine: The Forgotten Medicine

What You Need to Know About Folate and B12

 
Print Page
Email Article

Rapid Methods for Identification of Poliovirus Isolates and Determination of Polio Neutralizing Antibody Titers in Human Sera

  [ 40 votes ]   [ Discuss This Article ]
www.ProHealth.com • March 11, 2002




J Virol Methods 2002 Mar;101(1-2):189-96

Di Lonardo A, Buttinelli G, Amato C, Novello F, Ridolfi B, Fiore L.

Laboratory of Virology, Istituto Superiore di Sanita', Viale Regina Elena 299, 00161, Rome, Italy

A new rapid method for identification and determination of the titer of polioviruses and other enteroviruses in cell monolayers grown in microtiter plates is described. The method is based on immunoperoxidase staining of infected cells with commercial monoclonal antibodies (MAb) and biotin-labeled secondary antibody.

The presence of poliovirus or other enteroviruses was established as the appearance of at least one focus of cells with stained cytoplasm 6 h post-infection. Viral titers determined by this method were expressed as focus forming unit (FFU) per ml which was found to correspond approximately to 10 TCID(50)/ml. The suitability of this technique to determine poliovirus antibody titers in human sera was also tested comparing the immunocytochemical neutralization assay with a conventional neutralization in microtiter plates.

The test was standardized using reference human sera in order to produce antibody titers expressed in international units (IU). In addition to high reproducibility, the new neutralization test appears to be sensitive, specific and rapid, and might thus represent a useful tool for the diagnosis of polio and other enterovirus infections.

PMID: 11849697 [PubMed - in process]


Please Discuss This Article:   Post a Comment 



[ Be the first to comment on this article ]




 
Free Chronic Fatigue Syndrome and Fibromyalgia Newsletters
Subscribe to
Our FREE
Newsletter
Subscribe Now!
Receive up-to-date ME/CFS & Fibromyalgia treatment and research news
 Privacy Guaranteed  |  View Archives

Save on Immune Support Supplements

Featured Products

Optimized Curcumin Longvida® by ProHealth Optimized Curcumin Longvida® by ProHealth
Supports Cognition, Memory & Overall Health
FibroSleep™ by ProHealth FibroSleep™ by ProHealth
The All-in-One Natural Sleep Aid
Mitochondria Ignite™ with NT Factor® Mitochondria Ignite™ with NT Factor®
Reduce Fatigue up to 45%
Ultra ATP+, Double Strength Ultra ATP+, Double Strength
Get energized with malic acid & magnesium
Ultra EPA  - Fish Oil Ultra EPA - Fish Oil
Ultra concentrated source of essential fish oils

Natural Remedies

Protecting Our Eyes Against Time-Induced Damage Protecting Our Eyes Against Time-Induced Damage
Sleep Like a Baby in Nature's Cradle Sleep Like a Baby in Nature's Cradle
Dreaming of a Good Night's Sleep? Dreaming of a Good Night's Sleep?
Could a B-12 Deficiency Be Causing Your Symptoms? Could a B-12 Deficiency Be Causing Your Symptoms?
Protect Against Sun-Induced Skin Aging From The Inside Out Protect Against Sun-Induced Skin Aging From The Inside Out

FIBROMYALGIA RESOURCES
What is Fibromyalgia?
Fibromyalgia 101
Fibromyalgia Symptoms
Fibromyalgia Treatments
| CFS RESOURCES
What is CFS?
ME/CFS 101
ME/CFS Symptoms
ME/CFS Treatments
| FORUMS
Fibromyalgia
ME/CFS
ADVANCED MEDICAL LABS
WHOLESALE  |  AFFILIATES
GUARANTEE
CONTACT US
PRIVACY
RSS
SITE MAP
ProHealth on Facebook  ProHealth on Twitter  ProHealth on Pinterest  ProHealth on Google Plus
Credit Card Processing