ProHealth health Vitamin and Natural Supplement Store and Health
Home  |  Log In  |  My Account  |  View Cart  View Your ProHealth Vitamin and Supplement Shopping Cart
800-366-6056  |  Contact Us  |  Help
Facebook Google Plus
Fibromyalgia  Chronic Fatigue Syndrome & M.E.  Lyme Disease  Natural Wellness  Supplement News  Forums  Our Story
Store     Brands   |   A-Z Index   |   Best Sellers   |   New Products   |   Deals & Specials   |   Under $10   |   SmartSavings Club

Trending News

Prevent Or Reverse Diabetes: An Amazing Herbal Intervention

Exercise and Vitamin D Better Together for Heart Health

VIDEO: Essential Oils for Weight Loss

Can Ginkgo Give Your Brain a Boost?

Can Valerian Root Help You Sleep Better?

Fighting Statin-Induced Diabetes with CoQ10

Eight servings of veggies a day is clearly best for the heart

The Many Wonders of Calming Sandalwood Oil

Curcumin — A Novel Treatment Alternative for Depression

How Glycation Accelerates Aging

 
Print Page
Email Article

A Possible New Way to Block HIV Infection of Healthy Cells Discovered

  [ 18 votes ]   [ Discuss This Article ]
www.ProHealth.com • May 15, 2002




CHAMPAIGN, Ill. — An experiment to understand how chemokine peptides dock to a receptor on a cell wall – a pivotal connection that allows HIV to infect healthy cells – has yielded an unexpected fundamental discovery and a possible new way to block AIDS.

“There is still a lot to be done, but our findings provide new insights about the receptor’s flexibility and the potential mechanism of the interaction with HIV at the entry point of healthy cells,” said Ziwei Huang, a professor of biochemistry at the University of Illinois at Urbana-Champaign.

Huang was the principal investigator of the study, which appears in the May 17 issue of the Journal of Biological Chemistry. Huang’s team included researchers at the Kimmel Cancer Center at Thomas Jefferson University in Philadelphia and Dana-Farber Cancer Institute at the Harvard Medical School in Boston.

Researchers used computer modeling to predict the structure of chemokine receptor-ligand bonding. Then they synthesized reverse-chirality D-peptides, based on a portion of amino-acid sequences of two natural L-proteins that bond to CXCR4, a chemokine receptor to HIV discovered in the mid-1990s. CXCR4 and CD4, a receptor identifed in the 1980s, are thought to act together to let HIV bond and insert its replicating machinery into cells.

Only in rare cases – and never involving membrane-class proteins – have reverse-chirality peptides bound at identical receptors. “We didn’t have any expectation that our synthesized peptide would bind to the CXCR4 receptor,” Huang said. “If you switch the chirality, you have a mirror-image change. You’d expect a different lineup of key amino-acid sidechains. We looked at this out of pure curiosity. We just wanted to see what would happen.

“Unexpectedly,” he said, “these D-peptides displayed strong binding and antagonistic activity toward CXCR4, thus revealing that the peptide binding site on CXCR4 is tolerant of changes in the chirality of ligands,” Huang said. “Normally, ligand receptor-bindings are very specific, just like putting your left hand in your left pants pocket.”

Not only were the D-peptides still recognized, they fit into the receptor more snugly than their natural L-peptide counterparts, blocking the virus from bonding. Subsequent experiments, documented in the paper, showed the synthetic D-peptides have a longer half life, making them less susceptible to biological decay than L-peptides.

In making the discovery, Huang designed and synthesized the D-peptide and tested its bonding to the receptor at Illinois. Testing against HIV-1 was done at Dana-Farber in Dr. Joseph Sodroski’s laboratory and by a group led by Hideko Kaji at Jefferson Medical College, where Huang had previously worked and had begun his research.

Traditional drug therapies have targeted protease or reverse transcriptase activity that occur after HIV has entered healthy cells. None stop HIV from docking with cells.

“Researchers realize that this approach is not enough,” Huang said. “It would be nice to have other targets. Our discovery suggests the possibility of a whole new therapeutic weapon, which alone or in combination with existing therapies could work better to block AIDS.”


Illinois authors on the paper, along with Huang, were James Pesavento, Chang-Zhi Dong, Youli Wang and Jing An. Co-authors from Thomas Jefferson University Medical College were Kaji, Naiming Zhou, Zhaowen Luo, Jiansong Luo, Xuejun Fan, Dongxiang Liu, Xiaobing Han and Megumi Hiraoka. Dana-Farber researchers were Mark Cayabyab and Sodroski.

The National Institutes of Health, Center for AIDS Research, G. Harold and Leila Y. Mathers Charitable Foundation, William F. McCarty-Cooper, Douglas and Judity Krupp, and Japan Science Foundation funded the research.




Post a Comment

Featured Products From the ProHealth Store
FibroSleep™ Mitochondria Ignite™ with NT Factor® Vitamin D3 Extreme™

Looking for Vitamins, Herbs and Supplements?
Search the ProHealth Store for Hundreds of Natural Health Products


Article Comments



Be the first to comment on this article!

Post a Comment


 
NAD+ Ignite with Niagen

Featured Products

Ultra ATP+, Double Strength Ultra ATP+, Double Strength
Get energized with malic acid & magnesium
FibroSleep™ FibroSleep™
The All-in-One Natural Sleep Aid
Optimized Curcumin Longvida® Optimized Curcumin Longvida®
Supports Cognition, Memory & Overall Health
Mitochondria Ignite™ with NT Factor® Mitochondria Ignite™ with NT Factor®
Reduce Fatigue up to 45%
Energy NADH™ 12.5mg Energy NADH™ 12.5mg
Improve Energy & Cognitive Function

Natural Remedies

Astaxanthin - A Little-Known but Power-Packed Nutrient Astaxanthin - A Little-Known but Power-Packed Nutrient
Undenatured Type II Collagen - Chicken Soup for Your Joints Undenatured Type II Collagen - Chicken Soup for Your Joints
Live Without Anxiety or Stress Live Without Anxiety or Stress
Repair Damaged Mitochondria and Reduce Fatigue Up to 45% Repair Damaged Mitochondria and Reduce Fatigue Up to 45%
D-ficient? Health Risks You Need to Know About D-ficient? Health Risks You Need to Know About

CONTACT US
ProHealth, Inc.
555 Maple Ave
Carpinteria, CA 93013
(800) 366-6056  |  Email

· Become a Wholesaler
· Vendor Inquiries
· Affiliate Program
SHOP WITH CONFIDENCE
Credit Card Processing
SUBSCRIBE TO OUR NEWSLETTERS
Get the latest news about Fibromyalgia, M.E/Chronic Fatigue Syndrome, Lyme Disease and Natural Wellness

CONNECT WITH US ProHealth on Facebook  ProHealth on Twitter  ProHealth on Pinterest  ProHealth on Google Plus

© 2017 ProHealth, Inc. All rights reserved. Pain Tracker App  |  Store  |  Customer Service  |  Guarantee  |  Privacy  |  Contact Us  |  Library  |  RSS  |  Site Map