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Abstract: Urinary electrophoretic profiles from chronic fatigue syndrome and chronic fatigue syndrome/fibromyalgia patients: a pilot study for achieving their normalization

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www.ProHealth.com • December 28, 2004


J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Jan 5;814(1):43-51. Casado B, Zanone C, Annovazzi L, Iadarola P, Whalen G, Baraniuk JN. Department of Biochemistry "A. Castellani", University of Pavia, V.le Taramelli 3/B, 27100 Pavia, Italy; Division of Rheumatology, Immunology and Allergy, Department of Medicine, Georgetown University, 3800 Reservoir Road, LL Kober-Cogan, N.W., Washington, DC 20057-2197, USA. Aim of our study was to determine if there were distinct, disease-related patterns of urinary analytes in chronic fatigue syndrome (CFS) and chronic fatigue syndrome/fibromyalgia (CFS/FM) compared to normal controls (NC). Urine was collected from these subjects for two consecutive 24h periods and aliquots were submitted to micellar electrokinetic chromatography (MEKC). To compensate for the differences in peak migration times, these were normalized from the 35min duration of run to a 100-point scale, and each peak was assigned its normalized time measure. Peak heights were also normalized by dividing the mAU by that of the internal standard (creatinine) and multiplying by 100. MEKC with normalization for peak height and migration time generated comparable results within each of the patient groups. CFS/FM and CFS had significant differences in peaks compared to NC that may be of significance as biomarkers of illnesses. PMID: 15607706 [PubMed - as supplied by publisher]




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