ProHealth health Vitamin and Natural Supplement Store and Health
Home  |  Log In  |  My Account  |  View Cart  View Your ProHealth Vitamin and Supplement Shopping Cart
800-366-6056  |  Contact Us  |  Help
Facebook Google Plus
Fibromyalgia  Chronic Fatigue Syndrome & M.E.  Lyme Disease  Natural Wellness  Supplement News  Forums  Our Story
Store     Brands   |   A-Z Index   |   Best Sellers   |   New Products   |   Deals & Specials   |   Under $10   |   SmartSavings Club

Trending News

Ultrasound Therapy for Fibromyalgia and Lyme Disease

Curcumin: The All In One Solution, Part 2

What Are the Benefits of Vitamin K2?

Vitamin D deficiency + high fat diet = metabolic syndrome

Why You Should Take Your Apple Cider Vinegar at Night

Use Burdock Oil to Promote Healthy Hair Growth

AMA journal associates iron deficiency with hearing loss

Meet Your Weight Loss Goals

People with forms of early-onset Parkinson's disease may benefit from boosting niacin in diet, resea...

Lutein linked to preservation of crystallized intelligence

 
Print Page
Email Article

Characterization of XMRV in prostate cancer – Source: 2010 Genitourinary Cancers Symposium (Abstract 117), Mar 5-7, 2010

  [ 65 votes ]   [ Discuss This Article ]
By EA Klien, et al. • www.ProHealth.com • March 8, 2010


[Note: See also “ASCO GU: Novel Retrovirus Mimics HIV Transmission,” MedPageToday, Mar 6.]

Background: Xenotropic Murine Leukemia Related Virus (XMRV) is a novel gammaretrovirus discovered in prostate tissue of men genetically predisposed to prostate cancer. A growing body of evidence suggests a potential causative role in prostate cancer.

Methods: XMRV was isolated and cloned from prostate tissue of unselected men with prostate cancer who underwent radical prostatectomy, with initial identification accomplished by hybridization to a DNA microarray containing conserved sequences of known viruses. Live virus was injected intravenously into rhesus macaques for acute and chronic infection studies. Tissue distribution of XMRV was studied by immunohistochemistry (using a rhesus-derived monoclonal to ENV protein) and RT-PCR based assays. Effect of androgen on XMRV growth was assessed by infection of LnCap and DU145 cells using virus mutated in the androgen response element (ARE) promoter region as control. Chromosomal integration sites were mapped from human prostates using a PCR-based approached that produced biotinylated-ds DNA and isolated by binding to streptavidin-agarose Dynabeads. Expressed prostatic secretions (EPS) were obtained by milking radical prostatectomy specimens. The presence of XMRV in EPS was assessed by qRT-PCR.

Results:

1. Preliminary observations suggest that XMRV is detectable by immunohistochemistry in prostate epithelium;

2. XMRV preferentially integrates into host chromosomes at transcriptionally active sites;

3. IV injection of XMRV produces viremia and persistent infection in rhesus macaques with a robust immune response;

4. The XMRV promoter contains a consensus ARE;

5. Androgen stimulates transcription and replication of XMRV independent of cell growth;

6. XMRV is present in 15% of EPS from unselected cases of prostate cancer;

7. Human semen and semen-derived enhancers substantially increase XMRV in both prostate stroma and epithelium.

Conclusions:


• XMRV is infective in primates and produces an immune response.

• The presence of XMRV in EPS and effect of semen on infectivity suggest sexual transmission.

• The presence of an ARE and the stimulatory effect of androgen suggests that XMRV integration into host DNA could impart androgen stimulation on cellular genes, serving as a potential oncogenic mechanism.

Source: 2010 Genitourinary Cancers Symposium (Abstract 117), Mar 5-7, 2010. Klein EA, Villinger F, Das Gupta J, Magi-Galluzzi C, Hong S, Nguyen C, Weight CJ, Schochetman G, Hackett J, Silverman R. Cleveland Clinic Foundation, Cleveland, OH; Yerkes Primate Center, Atlanta, GA; Cleveland Clinic, Cleveland, OH; Abbott Diagnostics, Abbott Park, IL.





Post a Comment

Featured Products From the ProHealth Store
Ultra ATP+, Double Strength Ultra EPA  - Fish Oil Vitamin D3 Extreme™

Looking for Vitamins, Herbs and Supplements?
Search the ProHealth Store for Hundreds of Natural Health Products


Article Comments



Be the first to comment on this article!

Post a Comment


 
NAD+ Ignite with Niagen

Featured Products

Ultra EPA  - Fish Oil Ultra EPA - Fish Oil
Ultra concentrated source of essential fish oils
Energy NADH™ 12.5mg Energy NADH™ 12.5mg
Improve Energy & Cognitive Function
Ultra ATP+, Double Strength Ultra ATP+, Double Strength
Get energized with malic acid & magnesium
Mitochondria Ignite™ with NT Factor® Mitochondria Ignite™ with NT Factor®
Reduce Fatigue up to 45%
Vitamin D3 Extreme™ Vitamin D3 Extreme™
50,000 IU Vitamin D3 - Prescription Strength

Natural Remedies

Natural Bladder Control, Go Less and Live More Natural Bladder Control, Go Less and Live More
Soothe, Heal and Regulate Your Digestive System with Nutrient-Rich Aloe Vera Soothe, Heal and Regulate Your Digestive System with Nutrient-Rich Aloe Vera
Strontium - The Missing Mineral for Strong Bones Strontium - The Missing Mineral for Strong Bones
Coconut Oil - Healthy Gifts from the 'Tree of Life' Coconut Oil - Healthy Gifts from the 'Tree of Life'
Aching Muscles? Top 10 Nutrients to Take Back Your Life Aching Muscles? Top 10 Nutrients to Take Back Your Life

CONTACT US
ProHealth, Inc.
555 Maple Ave
Carpinteria, CA 93013
(800) 366-6056  |  Email

· Become a Wholesaler
· Vendor Inquiries
· Affiliate Program
SHOP WITH CONFIDENCE
Credit Card Processing
SUBSCRIBE TO OUR NEWSLETTERS
Get the latest news about Fibromyalgia, M.E/Chronic Fatigue Syndrome, Lyme Disease and Natural Wellness

CONNECT WITH US ProHealth on Facebook  ProHealth on Twitter  ProHealth on Pinterest  ProHealth on Google Plus

© 2017 ProHealth, Inc. All rights reserved. Pain Tracker App  |  Store  |  Customer Service  |  Guarantee  |  Privacy  |  Contact Us  |  Library  |  RSS  |  Site Map