ProHealth health Vitamin and Natural Supplement Store and Health
Home  |  Log In  |  My Account  |  View Cart  View Your ProHealth Vitamin and Supplement Shopping Cart
800-366-6056  |  Contact Us  |  Help
Facebook Google Plus
Fibromyalgia  Chronic Fatigue Syndrome & M.E.  Lyme Disease  Natural Wellness  Supplement News  Forums  Our Story
Store     Brands   |   A-Z Index   |   Best Sellers   |   New Products   |   Deals & Specials   |   Under $10   |   SmartSavings Club

Trending News

Fighting Heartburn and Gerd Naturally – And Safely!

Vital Molecule Increases Cellular Energy and Improves Cognitive Function

Natural Bladder Control, Go Less and Live More

Top Vitamin and Mineral Deficiencies — Are You at Risk?

Trimming the spare tire: Canola oil may cut belly fat

How Pomegranate May Protect Against Cancer

Omega Fix for Obesity: How the Right Fats Fight Fat

Curcumin Reverses the Cellular Damage of Chronic Stress

Probiotics improve cognition in Alzheimer's patients

The Onion: Cancer Fighter and Food Preserver

 
Print Page
Email Article

Characterization of XMRV in prostate cancer – Source: 2010 Genitourinary Cancers Symposium (Abstract 117), Mar 5-7, 2010

  [ 65 votes ]   [ Discuss This Article ]
By EA Klien, et al. • www.ProHealth.com • March 8, 2010


[Note: See also “ASCO GU: Novel Retrovirus Mimics HIV Transmission,” MedPageToday, Mar 6.]

Background: Xenotropic Murine Leukemia Related Virus (XMRV) is a novel gammaretrovirus discovered in prostate tissue of men genetically predisposed to prostate cancer. A growing body of evidence suggests a potential causative role in prostate cancer.

Methods: XMRV was isolated and cloned from prostate tissue of unselected men with prostate cancer who underwent radical prostatectomy, with initial identification accomplished by hybridization to a DNA microarray containing conserved sequences of known viruses. Live virus was injected intravenously into rhesus macaques for acute and chronic infection studies. Tissue distribution of XMRV was studied by immunohistochemistry (using a rhesus-derived monoclonal to ENV protein) and RT-PCR based assays. Effect of androgen on XMRV growth was assessed by infection of LnCap and DU145 cells using virus mutated in the androgen response element (ARE) promoter region as control. Chromosomal integration sites were mapped from human prostates using a PCR-based approached that produced biotinylated-ds DNA and isolated by binding to streptavidin-agarose Dynabeads. Expressed prostatic secretions (EPS) were obtained by milking radical prostatectomy specimens. The presence of XMRV in EPS was assessed by qRT-PCR.

Results:

1. Preliminary observations suggest that XMRV is detectable by immunohistochemistry in prostate epithelium;

2. XMRV preferentially integrates into host chromosomes at transcriptionally active sites;

3. IV injection of XMRV produces viremia and persistent infection in rhesus macaques with a robust immune response;

4. The XMRV promoter contains a consensus ARE;

5. Androgen stimulates transcription and replication of XMRV independent of cell growth;

6. XMRV is present in 15% of EPS from unselected cases of prostate cancer;

7. Human semen and semen-derived enhancers substantially increase XMRV in both prostate stroma and epithelium.

Conclusions:


• XMRV is infective in primates and produces an immune response.

• The presence of XMRV in EPS and effect of semen on infectivity suggest sexual transmission.

• The presence of an ARE and the stimulatory effect of androgen suggests that XMRV integration into host DNA could impart androgen stimulation on cellular genes, serving as a potential oncogenic mechanism.

Source: 2010 Genitourinary Cancers Symposium (Abstract 117), Mar 5-7, 2010. Klein EA, Villinger F, Das Gupta J, Magi-Galluzzi C, Hong S, Nguyen C, Weight CJ, Schochetman G, Hackett J, Silverman R. Cleveland Clinic Foundation, Cleveland, OH; Yerkes Primate Center, Atlanta, GA; Cleveland Clinic, Cleveland, OH; Abbott Diagnostics, Abbott Park, IL.





Post a Comment

Featured Products From the ProHealth Store
FibroSleep™ Mitochondria Ignite™ with NT Factor® Ultra EPA  - Fish Oil


Article Comments



Be the first to comment on this article!

Post a Comment


 
Natural Pain Relief Supplements

Featured Products

Energy NADH™ 12.5mg Energy NADH™ 12.5mg
Improve Energy & Cognitive Function
Mitochondria Ignite™ with NT Factor® Mitochondria Ignite™ with NT Factor®
Reduce Fatigue up to 45%
Ultra ATP+, Double Strength Ultra ATP+, Double Strength
Get energized with malic acid & magnesium
Optimized Curcumin Longvida® Optimized Curcumin Longvida®
Supports Cognition, Memory & Overall Health
FibroSleep™ FibroSleep™
The All-in-One Natural Sleep Aid

Natural Remedies

Everything You Always Wanted to Know About Sleep But Were Too Tired to Ask Everything You Always Wanted to Know About Sleep But Were Too Tired to Ask
The Surprising Benefits of Probiotics - What You Didn't Know The Surprising Benefits of Probiotics - What You Didn't Know
More Weight Loss than Any Other Discovery in Supplement History More Weight Loss than Any Other Discovery in Supplement History
Natural Support for Mood, Sleep and Mental Focus? L-theanine Natural Support for Mood, Sleep and Mental Focus? L-theanine
How to Jump-start and Sustain Energy Production in CFS How to Jump-start and Sustain Energy Production in CFS

CONTACT US
ProHealth, Inc.
555 Maple Ave
Carpinteria, CA 93013
(800) 366-6056  |  Email

· Become a Wholesaler
· Vendor Inquiries
· Affiliate Program
SHOP WITH CONFIDENCE
Credit Card Processing
SUBSCRIBE TO OUR NEWSLETTERS
Get the latest news about Fibromyalgia, M.E/Chronic Fatigue Syndrome, Lyme Disease and Natural Wellness

CONNECT WITH US ProHealth on Facebook  ProHealth on Twitter  ProHealth on Pinterest  ProHealth on Google Plus

© 2016 ProHealth, Inc. All rights reserved. Pain Tracker App  |  Store  |  Customer Service  |  Guarantee  |  Privacy  |  Contact Us  |  Library  |  RSS  |  Site Map