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Consensus at World Retrovirus Meeting in Prague - Replication Study Finding XMRV in CFS Just Matter of Time

  [ 88 votes ]   [ 5 Comments ]
www.ProHealth.com • May 4, 2010


The following Dutch press release was posted in English May 3 by the European Society for ME (http://esme-eu.com)

Last October U.S. scientists presented a breakthrough around the research on chronic fatigue syndrome (CFS), which was published in Science. They found traces of the retrovirus XMRV in the blood of CFS patients. Thereafter, three groups of European researchers, including a Dutch group from Nijmegen, couldn’t confirm these findings. However, at the 'Centennial Retrovirus Meeting' in Prague it became clear that the first positive 'replication study' seems only a matter of time. [Abstracts of papers presented at the Prague conference are available at the CRM site.]

The June issue of the Dutch magazine Ortho will focus on the multi-day conference in Prague, which ends today. Especially in the corridors this new retrovirus was the talk of the day. Insiders agree that the negative XMRV studies which have been published so far, were not exact replication studies. The several groups of researchers used techniques that differed too much from those used by the U.S. researchers. This is also true for a yet unpublished German study, where XMRV wasn’t found in blood samples from CFS patients either.

Recently the American scientist Dr. Judy Mikovits visited several European research groups to help them with the proper laboratory technique. It is now clear that these visits are starting to pay off.

During the Prague Conference, Mikovits explained once more in great detail the complex methodology of the Whittemore-Peterson Institute (WPI), the National Cancer Institute (NCI) and the Cleveland Clinic. This methodology of culturing the virus is imperative because XMRV is only present in extremely low concentrations in the peripheral blood.

Dr. Francis Ruscetti of the renowned NCI – a U.S. government agency – told Ortho that he hopes this controversy will all die away in 2011.

He is especially surprised about the fact that the investigators of the UMC St. Radboud at Nijmegen concealed in their publication that the Americans found traces of XMRV in the same blood samples from the Dutch patients. "I don’t know how they get away ethically with this," said Ruscetti. "I don’t think that is good science."

Ruscetti pointed out again that the WPI, the NCI and the Cleveland Clinic applied four procedures in their research. "In those negative studies they only tried one." Ruscetti also ventilated his annoyance over what he calls the "whispering campaign" about contamination. According to the Nijmegen researchers, the Americans contaminated or polluted the Dutch blood samples.

Among others Ruscetti is supported by Prof. Dr. John Coffin, who is linked both to the NCI and Tufts University in Boston. He is considered one of the most prominent retrovirologists in the world.

"People have raised the issue of contamination," said Coffin. "But we don’t really know anything about that yet. We don’t have any evidence. A lot of the studies were done in fact at the NCI, in the lab of Francis and Sandra Ruscetti. They have a long experience with these viruses and are very careful workers."

Coffin emphasized once again that doing a replication study implies that it is performed in exactly the same way. "In none of the studies that have been published so far that were negative, the virus was cultured," said Coffin. "Only the Science study did this, which is a very compelling point."

Researchers from Nijmegen were not present at this leading conference.




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Article Comments Post a Comment

XMRV Replication
Posted by: Mya Symons
May 4, 2010
I have been reading some about XMRV. It is my understanding that some scientists think, for the most part, that XMRV replicates primarily in the lymph nodes and sex organs and not the blood. Therefore, sometimes it can be difficult to find enough in the blood to make a test positive. That is why it is more effective to culture and grow the virus. Do I have that right? I have a question about that. What is a PCR and how does it work? If the virus is only in minute amounts in the blood, is it likely this PCR test will give a false negative?
Reply Reply

XMRV hard to find in blood
Posted by: oerganix
May 4, 2010
Yes, you are right, XMRV is hard to find in blood. I can't explain PCR but it is my understanding that it would be fairly easy to get a false negative. VIP is no longer doing the same type of test they did at first and they have said they will retest the negative samples at some point, with their more reliable test(s) as they are developed. Apparently, the retrovirus has to be cultivated carefully to bring its numbers up to where they can be found, and this takes quite a lot of time. This is what the Failure To Find studies did not do, among other things.
Reply Reply

PCR
Posted by: IanH
May 5, 2010
PCR = Polymerase chain Reaction. This method uses an enzyme, usually DNA polymerase to make many copies of the DNA fragment so that it can be detected. Of course XMRV is an RNA virus so the RNA of the virus is copied into DNA and it is the DNA that is detected in the PCR.
Reply Reply

 
XMRV and PCR
Posted by: Mya Symons
May 7, 2010
Regarding---"XMRV is an RNA virus so the RNA of the virus is copied into DNA and it is the DNA that is detected in the PCR." One more question if you don't mind. You seem to know a lot about PCR. Is it possible for the evidence of the change to the DNA due to the RNA copy to be found in one area of the body and not the other? Would you always find evidence of any retrovirus in one sample of blood once the RNA is copied into DNA?

 

 
XMRV and PCR
Posted by: Mya Symons
May 7, 2010
Regarding---"XMRV is an RNA virus so the RNA of the virus is copied into DNA and it is the DNA that is detected in the PCR." One more question if you don't mind. You seem to know a lot about PCR. Is it possible for the evidence of the change to the DNA due to the RNA copy to be found in one area of the body and not the other? Would you always find evidence of any retrovirus in one sample of blood once the RNA is copied into DNA?

 



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