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A novel real-time PCR system for simultaneous detection of human viruses in clinical samples from patients with uncertain diagnoses – Source: Journal of Medical Virology, Feb 2011

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By Harutaka Katano, et al • www.ProHealth.com • December 27, 2010


A novel simultaneous detection system for human viruses was developed using a real-time polymerase chain reaction (PCR) system to identify causes of infection in clinical samples from patients with uncertain diagnoses.

This system, designated as the "multivirus real-time PCR," has the potential to detect 163 human viruses (47 DNA viruses and 116 RNA viruses) in a 96-well plate simultaneously.

The specificity and sensitivity of each probe-primer set were confirmed with cells or tissues infected with specific viruses.

The multivirus real-time PCR system:

• Showed profiles of virus infection in 20 autopsies of acquired immunodeficiency syndrome patients,

• And detected frequently TT virus, cytomegalovirus, human herpesvirus 6, and Epstein-Barr virus in various organs;

• However, RNA viruses were detected rarely except for human immunodeficiency virus-1.

Pathology samples from 40 patients with uncertain diagnoses were examined, including cases of encephalitis, hepatitis, and myocarditis.

Herpes simplex virus 1, human herpesvirus 6, and parechovirus 3 were identified as causes of diseases in four cases of encephalitis,

• While no viruses were identified in other cases as causing disease.

This multivirus real-time PCR system can be useful for detecting virus in specimens from patients with uncertain diagnoses.

Source: Journal of Medical Virology, Feb 2011;83(2):322-30. PMID: 21181930 by Katano H, Kano M, Nakamura T, Kanno T, Asanuma H, Sata T. Department of Pathology, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan. [Email:  katano@nih.go.jp]





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