Note: The abstract of this report by cancer researchers Bryan Danielson, Gustavo Ayala, and Jason Kimata at Baylor College of Medicine, published online Nov 15, 2010 by the Journal of Infectious Diseases is provided below. It concludes "the presence of XMRV in normal tissue suggests that infection may precede cancer onset."
To read the free full text of the article go to: http://jid.oxfordjournals.org/content/202/10/1470.long
Title: Detection of Xenotropic Murine Leukemia Virus-Related Virus in Normal and Tumor Tissue of Patients from the Southern United States with Prostate Cancer is Dependent on Specific Polymerase Chain Reaction Conditions
Background: There are questions regarding the prevalence of xenotropic murine leukemia virus-related virus (XMRV) in patients with prostate cancer and its association with the RNASEL R462Q polymorphism. [This gene variant has been associated with increased susceptibility to prostate cancer.]
We therefore investigated whether XMRV infection could be found in patients with prostate cancer from the southern United States, and we sought to verify the association with the R462Q.
Methods: Prostate tissue specimens of 144 patients with prostate cancer from the southern United States were genotyped for R462Q by real time polymerase chain reaction allelic discrimination and were screened for XMRV proviral DNA by nested polymerase chain reaction specific for the env gene.
• The R462Q polymorphism was found at an allelic frequency of 0.33.
• XMRV was detected in 32 (22%) of the 144 patients.
• Patients were significantly more likely to test positive for XMRV in both tumor and normal tissue rather than either alone (K = 0.64).
• A positive result for XMRV was not significantly correlated with the R462Q polymorphism (P = .82) or clinical pathological parameters of prostate cancer, including Gleason score (P = .29).
Conclusions: XMRV is detectable in normal and tumor prostate tissue from patients with prostate cancer, independent of R462Q. The presence of XMRV in normal tissue suggests that infection may precede cancer onset.
Source: Journal of Infectious Diseases, Nov 15, 2010. PMID: 20936978, by Bryan P. Danielson, Gustavo E. Ayala, and Jason T. Kimata. Departments of Molecular Virology and Microbiology and Pathology, and Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, Texas, USA. [Email: firstname.lastname@example.org]