ProHealth health Vitamin and Natural Supplement Store and Health
Home  |  Log In  |  My Account  |  View Cart  View Your ProHealth Vitamin and Supplement Shopping Cart
800-366-6056  |  Contact Us  |  Help
Facebook Google Plus
Fibromyalgia  Chronic Fatigue Syndrome & M.E.  Lyme Disease  Natural Wellness  Supplement News  Forums  Our Story
Store     Brands   |   A-Z Index   |   Best Sellers   |   New Products   |   Deals & Specials   |   Under $10   |   SmartSavings Club

Trending News

Can Autoimmune Conditions be Reversed? Researchers Make a Surprising Discovery

Scientifically-designed fasting diet lowers risks for major diseases

How One Tiny Molecule Turned into One Huge Health Breakthrough

Acupuncture boosts effectiveness of standard medical care for chronic pain, depression

Research on Astaxanthin Demonstrates Significant Whole Body Benefits

Humans have three times more brown body fat

Nutrients Boost Stem Cell Function

B12 Proven Essential for Every Cell

Soy isoflavones may benefit breast cancer patients

Dietary prebiotics improve sleep, buffer impacts of stress, says study

 
Print Page
Email Article

In Vitro Anti-HSV-1 Activities of d-lenolate at different concentrations

  [ Not Yet Rated ]   [ Discuss This Article ]
www.ProHealth.com • July 25, 2014


Source - The Science Behind d-Lanelate® Studies 1999 - 2014. East Park™ Research

Objective

To determine the in vitro anti-HSV-1 activities of East Park Research’s d-lenolate at different concentrations.

Methods

Both 5% and 10% d-lenolate stock solutions were prepared by resuspending 2 grams and 4 grams of the powdered olive leaf extract in 40mL of PBS. The extract was vigorously vortexed and centrifuged to sediment under sterile conditions. The supernatants were used to test the antiviral activity of the desired compound. Ten-fold dilutions of each d-lenolate extract stock solution were prepared in PBS. Varying dilutions of HSV-1 were added to each of the dilutions of d-lenolate.

For the adsorption only tests 200uL of each mixture was adsorbed onto Vero cell monolayers for one hour. The mixture was aspirated prior to the addition of DMEM with 1% carboxymethylcellulose. Plates were incubated at 37C and 5% CO2 for 72 hours. Virus controls were maintained.

For the adsorption + infection tests, 200uL of the mixture containing DMEM with 1% carboxymethylcellulose was incubated at 37C and 5% CO2 for 72 hours. Virus controls were maintained.

The cells were fixed with for 1 hour; washed with PBS; histochemically stained with Vecta Stain (Vector Labs) according to manufacturer’s protocol.

The ability of different concentrations of d-lenolate extract to inhibit fusion of Vero cell monolayers caused by syncytial mutants HSV-1(oncsyn) and HSV-1(gK) were assessed by phase contrast microscopy. Confluent monolayers of cells were infected with an MOI of 10 PFU/cell, various concentrations of the d-lenolate was added to media immediately after adsorption, and fusion was assessed at 12-18 hours after infection.

Observations

For the adsorption experiments, using the 1% and 0.5% d-lenolate extract treatments, no viral Plaques were observed. A greater than 4 log reduction in the number of viral plaques was observed in the 0.25% treatment group. Approximately a 1.5 log reduction in plaques was seen in the 0.125% treatment group.

For the adsorption + infection experiments, d-lenolate extract was found to be toxic in the 1% and 0.5% treatment groups. At 0.25% an approximately 4 log reduction in the number of viral plaques was observed. Approximately a 1.5 log reduction in plaques was seen in the 0.125% treatment group.

For the fusion inhibition assay, d-lenolate inhibited the fusion activity of both the onccsyn and gK fusing viruses.

Conclusion

The d-lenolate solution demonstrated antiviral activity at higher concentrations. The effect decreased with increasing dilutions. The fusion activities of two fusing viruses were inhibited.

HSV-1

Adsorption only

Concentration Plaque#(dilution) Plaque#/mL Log difference from control
1% d-lenolate extract (10% stock) 0(10-3) 0 >5 logs
0.5% d-lenolate extract (10% stock) 0(10-3) 0 >5 logs
0.25% d-lenolate extract(10% stock) 11(10-4) 4.4 x 105 ~4 logs
0.5% d-lenolate extract(5% stock) 0(10-3) 0 > 5 logs
0.25% d-lenolate extract(5% stock) 6(10-4) 2.4 x 105 > 4 logs
0.125% d-lenolate extract(5% stock) 4(10-6) 1.6 x 107 ~1.5 logs
Control 14(10-8) 5.6 x 109 -----
 



HSV-1

Adsorption + Infection

Concentration Plaque#(dilution) Plaque#/mL Log difference from control
1% d-lenolate extract (10% stock) toxic 0 -----
0.5% d-lenolate extract (10% stock) toxic 0 >5 logs
0.25% d-lenolate extract(10% stock) 8(10-4) 3.2 x 105 ~4 logs
0.5% d-lenolate extract(5% stock) toxic 0 ----
0.25% d-lenolate extract(5% stock) 9(10-4) 3.6 x 105 ~4 logs
0.125% d-lenolate extract(5% stock) 7(10-6) 2.8 x 107 ~1.5 logs
Control 12(10-8) 4.8 x 109 ----
 
 




Post a Comment

Featured Products From the ProHealth Store
Ultra EPA  - Fish Oil Optimized Curcumin Longvida® Ultra ATP+, Double Strength

Looking for Vitamins, Herbs and Supplements?
Search the ProHealth Store for Hundreds of Natural Health Products


Article Comments



Be the first to comment on this article!

Post a Comment


 
NAD+ Ignite with Niagen

Featured Products

Optimized Curcumin Longvida® Optimized Curcumin Longvida®
Supports Cognition, Memory & Overall Health
FibroSleep™ FibroSleep™
The All-in-One Natural Sleep Aid
Ultra EPA  - Fish Oil Ultra EPA - Fish Oil
Ultra concentrated source of essential fish oils
Ultra ATP+, Double Strength Ultra ATP+, Double Strength
Get energized with malic acid & magnesium
Energy NADH™ 12.5mg Energy NADH™ 12.5mg
Improve Energy & Cognitive Function

Natural Remedies

Natural Relief for Soreness, Pain and Swelling - Putting Out the Fire Natural Relief for Soreness, Pain and Swelling - Putting Out the Fire
Energy Breakthrough - One Fibromyalgia Patient’s Fortuitous Discovery Energy Breakthrough - One Fibromyalgia Patient’s Fortuitous Discovery
Guarding Against the Dangers of Vitamin D Deficiency Guarding Against the Dangers of Vitamin D Deficiency
Red Yeast Rice - Natural Option for Supporting Healthy Cholesterol Red Yeast Rice - Natural Option for Supporting Healthy Cholesterol
Health Benefits Are Brewing in Green Tea Health Benefits Are Brewing in Green Tea

CONTACT US
ProHealth, Inc.
555 Maple Ave
Carpinteria, CA 93013
(800) 366-6056  |  Email

· Become a Wholesaler
· Vendor Inquiries
· Affiliate Program
SHOP WITH CONFIDENCE
Credit Card Processing
SUBSCRIBE TO OUR NEWSLETTERS
Get the latest news about Fibromyalgia, M.E/Chronic Fatigue Syndrome, Lyme Disease and Natural Wellness

CONNECT WITH US ProHealth on Facebook  ProHealth on Twitter  ProHealth on Pinterest  ProHealth on Google Plus

© 2017 ProHealth, Inc. All rights reserved. Pain Tracker App  |  Store  |  Customer Service  |  Guarantee  |  Privacy  |  Contact Us  |  Library  |  RSS  |  Site Map