ProHealth health Vitamin and Natural Supplement Store and Health
Log In  |  My Account  |  View Cart  View Your ProHealth Vitamin and Supplement Shopping Cart
800-366-6056  |  Contact Us  |  Help

|
|
|
|

Trending News

CDC Whistleblower Reveals Widespread Manipulation of Scientific Data and Top-Down Pressure on CDC Sc...

Do gut bacteria rule our minds?

Fatigue & Fibro Fog: Could You Have a B-12 Deficiency?

Irritable Bowel Syndrome: Unlocking the Secrets of Peppermint, Acacia and Fennel

9 Health Benefits of Cucumbers

Preserving Cognitive Function with Aging

Antibiotics in early life may alter immunity long-term

Coenzyme Q10 Benefits Symptoms in Gulf War Veterans: Results of a Randomized Double-Blind Study

Low vitamin D levels linked to increased risks after noncardiac surgery

Depression Linked to Parkinson's Disease

 
Print Page
Email Article

In Vitro Anti-HSV-1 Activities of d-lenolate at different concentrations

  [ Not Yet Rated ]   [ Discuss This Article ]
www.ProHealth.com • July 25, 2014


Source - The Science Behind d-Lanelate® Studies 1999 - 2014. East Park™ Research

Objective

To determine the in vitro anti-HSV-1 activities of East Park Research’s d-lenolate at different concentrations.

Methods

Both 5% and 10% d-lenolate stock solutions were prepared by resuspending 2 grams and 4 grams of the powdered olive leaf extract in 40mL of PBS. The extract was vigorously vortexed and centrifuged to sediment under sterile conditions. The supernatants were used to test the antiviral activity of the desired compound. Ten-fold dilutions of each d-lenolate extract stock solution were prepared in PBS. Varying dilutions of HSV-1 were added to each of the dilutions of d-lenolate.

For the adsorption only tests 200uL of each mixture was adsorbed onto Vero cell monolayers for one hour. The mixture was aspirated prior to the addition of DMEM with 1% carboxymethylcellulose. Plates were incubated at 37C and 5% CO2 for 72 hours. Virus controls were maintained.

For the adsorption + infection tests, 200uL of the mixture containing DMEM with 1% carboxymethylcellulose was incubated at 37C and 5% CO2 for 72 hours. Virus controls were maintained.

The cells were fixed with for 1 hour; washed with PBS; histochemically stained with Vecta Stain (Vector Labs) according to manufacturer’s protocol.

The ability of different concentrations of d-lenolate extract to inhibit fusion of Vero cell monolayers caused by syncytial mutants HSV-1(oncsyn) and HSV-1(gK) were assessed by phase contrast microscopy. Confluent monolayers of cells were infected with an MOI of 10 PFU/cell, various concentrations of the d-lenolate was added to media immediately after adsorption, and fusion was assessed at 12-18 hours after infection.

Observations

For the adsorption experiments, using the 1% and 0.5% d-lenolate extract treatments, no viral Plaques were observed. A greater than 4 log reduction in the number of viral plaques was observed in the 0.25% treatment group. Approximately a 1.5 log reduction in plaques was seen in the 0.125% treatment group.

For the adsorption + infection experiments, d-lenolate extract was found to be toxic in the 1% and 0.5% treatment groups. At 0.25% an approximately 4 log reduction in the number of viral plaques was observed. Approximately a 1.5 log reduction in plaques was seen in the 0.125% treatment group.

For the fusion inhibition assay, d-lenolate inhibited the fusion activity of both the onccsyn and gK fusing viruses.

Conclusion

The d-lenolate solution demonstrated antiviral activity at higher concentrations. The effect decreased with increasing dilutions. The fusion activities of two fusing viruses were inhibited.

HSV-1

Adsorption only

Concentration Plaque#(dilution) Plaque#/mL Log difference from control
1% d-lenolate extract (10% stock) 0(10-3) 0 >5 logs
0.5% d-lenolate extract (10% stock) 0(10-3) 0 >5 logs
0.25% d-lenolate extract(10% stock) 11(10-4) 4.4 x 105 ~4 logs
0.5% d-lenolate extract(5% stock) 0(10-3) 0 > 5 logs
0.25% d-lenolate extract(5% stock) 6(10-4) 2.4 x 105 > 4 logs
0.125% d-lenolate extract(5% stock) 4(10-6) 1.6 x 107 ~1.5 logs
Control 14(10-8) 5.6 x 109 -----
 



HSV-1

Adsorption + Infection

Concentration Plaque#(dilution) Plaque#/mL Log difference from control
1% d-lenolate extract (10% stock) toxic 0 -----
0.5% d-lenolate extract (10% stock) toxic 0 >5 logs
0.25% d-lenolate extract(10% stock) 8(10-4) 3.2 x 105 ~4 logs
0.5% d-lenolate extract(5% stock) toxic 0 ----
0.25% d-lenolate extract(5% stock) 9(10-4) 3.6 x 105 ~4 logs
0.125% d-lenolate extract(5% stock) 7(10-6) 2.8 x 107 ~1.5 logs
Control 12(10-8) 4.8 x 109 ----
 
 



Please Discuss This Article:   Post a Comment 



[ Be the first to comment on this article ]




 
Free Chronic Fatigue Syndrome and Fibromyalgia Newsletters
Subscribe to
Our FREE
Newsletter
Subscribe Now!
Receive up-to-date ME/CFS & Fibromyalgia treatment and research news
 Privacy Guaranteed  |  View Archives

Save on Vitamin B-12

Save on Vitamins and Supplements

Featured Products

FibroSleep™ by ProHealth FibroSleep™ by ProHealth
The All-in-One Natural Sleep Aid
Optimized Curcumin Longvida® by ProHealth Optimized Curcumin Longvida® by ProHealth
Supports Cognition, Memory & Overall Health
Vitamin D3 Extreme™ by ProHealth Vitamin D3 Extreme™ by ProHealth
50,000 IU Vitamin D3 - Prescription Strength
Ultra EPA  - Fish Oil Ultra EPA - Fish Oil
Ultra concentrated source of essential fish oils
Energy NADH™ 12.5mg Energy NADH™ 12.5mg
Improve Energy & Cognitive Function

Natural Remedies

The Remarkable Benefits of Reishi Medicinal Mushrooms The Remarkable Benefits of Reishi Medicinal Mushrooms
Sleep Like a Baby in Nature's Cradle Sleep Like a Baby in Nature's Cradle
IBS, Crohn’s Disease, Colitis, and Other Digestive Disorders IBS, Crohn’s Disease, Colitis, and Other Digestive Disorders
Irritable Bowel Syndrome: Unlocking the Secrets of Peppermint, Acacia and Fennel Irritable Bowel Syndrome: Unlocking the Secrets of Peppermint, Acacia and Fennel
Front Line Defense Against Colds & Flu - Support for Healthy Immune System Balance Front Line Defense Against Colds & Flu - Support for Healthy Immune System Balance

FIBROMYALGIA RESOURCES
What is Fibromyalgia?
Fibromyalgia 101
Fibromyalgia Symptoms
Fibromyalgia Treatments
| CFS RESOURCES
What is CFS?
ME/CFS 101
ME/CFS Symptoms
ME/CFS Treatments
| FORUMS
Fibromyalgia
ME/CFS
ADVANCED MEDICAL LABS
WHOLESALE  |  AFFILIATES
GUARANTEE
CONTACT US
PRIVACY
RSS
SITE MAP
ProHealth on Facebook  ProHealth on Twitter  ProHealth on Pinterest  ProHealth on Google Plus
Credit Card Processing