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The persistence of spirochetal nucleic acids in active Lyme arthritis.

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By Bradley JF, Johnson RC, Goodman JL • www.ProHealth.com • March 15, 1994


Abstract

Six of seven patients with Lyme arthritis were positive by PCR. In contrast, all 18 synovial fluid samples from patients with other disorders, including rheumatoid arthritis, spondyloarthropathy, gout, pseudogout, hemarthrosis, degenerative joint disease, lupus, papillary synovitis, and trauma, were negative by PCR (P < 0.001, Lyme arthritis compared with controls, Fisher exact test). All 38 laboratory controls were negative by PCR. The assay reproducibly detected 20 or fewer B. burgdorferi cells directly or when added to extracted synovial fluid that was previously negative by PCR. Polymerase chain reaction was done four times with identical results, including analyses with both outer surface protein A primer sets.

Ann Intern Med. 1994 Mar 15;120(6):487-9. Case Reports; Research Support, U.S. Gov't, P.H.S.





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