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Lyme disease: a selective medium for isolation of the suspected etiological agent, a spirochete.

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By Johnson SE, Klein GC, Schmid GP, Bowen GS, Feeley JC, Schulze T • www.ProHealth.com • January 1, 1984


Abstract

A simple procedure with a new selective culture medium for the isolation of the suspected etiological agent of Lyme disease from ticks is described. Live ticks (Ixodes dammini) were ground with a mortar and pestle, and the suspensions were inoculated into a selective and nonselective medium. The selective medium, which contained kanamycin and 5-fluorouracil, yielded positive spirochete cultures from 100% of the pooled ticks and from 79% of the single tick specimens. The isolation rate for the nonselective medium was 0% from the tick pools and 58% from the single tick specimens.

J Clin Microbiol. 1984 Jan;19(1):81-2. Comparative Study





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