ProHealth health Vitamin and Natural Supplement Store and Health
Home  |  Log In  |  My Account  |  View Cart  View Your ProHealth Vitamin and Supplement Shopping Cart
800-366-6056  |  Contact Us  |  Help
Facebook Google Plus
Fibromyalgia  Chronic Fatigue Syndrome & M.E.  Lyme Disease  Natural Wellness  Supplement News  Forums  Our Story
Store     Brands   |   A-Z Index   |   Best Sellers   |   New Products   |   Deals & Specials   |   Under $10   |   SmartSavings Club

Trending News

B Vitamins May Protect Against Damaging Effects of Air Pollution, and Improve Cognition and Psychiat...

Relief for IBS, Colitis, Crohn’s And More

Can Magnesium Relieve Your Tinnitus?

Restless Leg Syndrome (RLS) - are you running a marathon each night?

Resveratrol Proven to Slow Brain Aging

Magnesium Protects Against Stroke, Heart Disease and Diabetes

Fruits and vegetables' latest superpower? Lowering blood pressure

Neroli Oil: The Pleasantly Fragrant Citrus Oil

Prenatal vitamin D could help prevent autism in children

Restoring NAD+ could reduce DNA damage accumulation

 
Print Page
Email Article

Propagation & characterization of human herpesvirus-7 (HHV-7) isolates in a continuous T-lymphoblastoid cell line (SupT1)

  [ 23 votes ]   [ Discuss This Article ]
www.ProHealth.com • August 2, 1998


After initial culture of HHV-7 in PHA-stimulated human cord
blood mononuclear cells (HCBMC), six HHV-7 isolates were
propagated successfully in an immature continuous
T-lymphoblastoid cell line SupT1. All six isolates infected
efficiently the SupT1 cells, and the infected cells became
grossly enlarged and multinucleated 7-21 days post-infection.
Various stages of HHV-7 morphogenesis were detected. Cell-free
supernatants from HHV-7-infected SupT1 cells were infectious
to HCBMC as well as to SupT1 cells. The HHV-7-infected SupT1
and HCBMC cell lysates contained more infectious virus than
the centrifuged cell culture fluid supernates from the same
culture. The HHV-7 isolates H7- 2, H7-3, JHC, and JB,
concentrated 500 times, had average infectivity titers of
10(3.0) TCID50/ml while strains H7-4 and KHR titered
approximately 1-2 logs higher.

When all six HHV-7 isolates were propagated in SupT1 and culture
fluid supernatants were examined 14-21 days post-infection by negative
stain electron microscopy they contained an average of 1.9 x 10(9)
virus particles/liter. IFA and ELISA, using HHV-7/SupT1 cell lysate
as an antigen, seem to correlate well in detecting high and
low HHV-7 antibody in sera from chronic fatigue patients and
healthy donors as controls. HHV-7 from SupT1 cell culture was
free of HHV-6 and other human herpesviruses as tested by PCR,
and the HHV-7 PCR signal was still strong when the viral
preparation was diluted to 4.82 x 10(2) genome copies. Since
HCBMC are expensive to obtain and available in only small
amounts, it is difficult to obtain large quantities of HHV-7
antigen. On the other hand, the SupT1 cell is an excellent
source to produce consistently sufficient quantities of HHV-7
for purification studies, development of immunodiagnostics, in
vivo infectivity studies, evaluation of antiviral drugs, and
molecular biological studies.

Ablashi DV, Handy M, Bernbaum J, Chatlynne LG, Lapps W, Kramarsky B, 




Post a Comment

Featured Products From the ProHealth Store
Optimized Curcumin Longvida® FibroSleep™ Mitochondria Ignite™ with NT Factor®

Looking for Vitamins, Herbs and Supplements?
Search the ProHealth Store for Hundreds of Natural Health Products


Article Comments



Be the first to comment on this article!

Post a Comment


 
NAD+ Ignite with Niagen

Featured Products

FibroSleep™ FibroSleep™
The All-in-One Natural Sleep Aid
Mitochondria Ignite™ with NT Factor® Mitochondria Ignite™ with NT Factor®
Reduce Fatigue up to 45%
Energy NADH™ 12.5mg Energy NADH™ 12.5mg
Improve Energy & Cognitive Function
Vitamin D3 Extreme™ Vitamin D3 Extreme™
50,000 IU Vitamin D3 - Prescription Strength
Ultra ATP+, Double Strength Ultra ATP+, Double Strength
Get energized with malic acid & magnesium

Natural Remedies

A Hard-Working Molecule that May Help Ease Pain & Brighten Mood A Hard-Working Molecule that May Help Ease Pain & Brighten Mood
Itching to Find Dry Skin Relief? Itching to Find Dry Skin Relief?
Aches and Pains? A Simple Solution You'll Love Aches and Pains? A Simple Solution You'll Love
Guarding Against the Dangers of Vitamin D Deficiency Guarding Against the Dangers of Vitamin D Deficiency
Fatigue & Fibro Fog: Could You Have a B-12 Deficiency? Fatigue & Fibro Fog: Could You Have a B-12 Deficiency?

CONTACT US
ProHealth, Inc.
555 Maple Ave
Carpinteria, CA 93013
(800) 366-6056  |  Email

· Become a Wholesaler
· Vendor Inquiries
· Affiliate Program
SHOP WITH CONFIDENCE
Credit Card Processing
SUBSCRIBE TO OUR NEWSLETTERS
Get the latest news about Fibromyalgia, M.E/Chronic Fatigue Syndrome, Lyme Disease and Natural Wellness

CONNECT WITH US ProHealth on Facebook  ProHealth on Twitter  ProHealth on Pinterest  ProHealth on Google Plus

© 2017 ProHealth, Inc. All rights reserved. Pain Tracker App  |  Store  |  Customer Service  |  Guarantee  |  Privacy  |  Contact Us  |  Library  |  RSS  |  Site Map