ProHealth health Vitamin and Natural Supplement Store and Health
Home  |  Log In  |  My Account  |  View Cart  View Your ProHealth Vitamin and Supplement Shopping Cart
800-366-6056  |  Contact Us  |  Help
Facebook Google Plus
Fibromyalgia  Chronic Fatigue Syndrome & M.E.  Lyme Disease  Natural Wellness  Supplement News  Forums  Our Story
Store     Brands   |   A-Z Index   |   Best Sellers   |   New Products   |   Deals & Specials   |   Under $10   |   SmartSavings Club

Trending News

10 Fibro-Friendly Foods with a Bonus: Beautiful Skin

Fight Back! Win the War Being Waged Against Your Immune System

Studies Show that Magnesium L-threonate Improves Brain Plasticity, Leading to Direct and Significant...

Clary Sage Oil May Be Pricey, but Its Benefits Are Priceless

Component of red wine, grapes can help to reduce inflammation, study finds

Poly MVA: A Novel Therapy for Increasing Energy, Repairing DNA, and Promoting Overall Health

Pumpkin Pie Turmeric Breakfast Smoothie - Vegan + Gluten-Free

Vitamin D supplementation extends life in mouse model of Huntington's disease

Omega-3 fatty acid stops known trigger of lupus

Conquer Your Email Inbox, Increase Productivity and Reduce Stress

Print Page
Email Article

Propagation & characterization of human herpesvirus-7 (HHV-7) isolates in a continuous T-lymphoblastoid cell line (SupT1)

  [ 23 votes ]   [ Discuss This Article ] • August 2, 1998

After initial culture of HHV-7 in PHA-stimulated human cord
blood mononuclear cells (HCBMC), six HHV-7 isolates were
propagated successfully in an immature continuous
T-lymphoblastoid cell line SupT1. All six isolates infected
efficiently the SupT1 cells, and the infected cells became
grossly enlarged and multinucleated 7-21 days post-infection.
Various stages of HHV-7 morphogenesis were detected. Cell-free
supernatants from HHV-7-infected SupT1 cells were infectious
to HCBMC as well as to SupT1 cells. The HHV-7-infected SupT1
and HCBMC cell lysates contained more infectious virus than
the centrifuged cell culture fluid supernates from the same
culture. The HHV-7 isolates H7- 2, H7-3, JHC, and JB,
concentrated 500 times, had average infectivity titers of
10(3.0) TCID50/ml while strains H7-4 and KHR titered
approximately 1-2 logs higher.

When all six HHV-7 isolates were propagated in SupT1 and culture
fluid supernatants were examined 14-21 days post-infection by negative
stain electron microscopy they contained an average of 1.9 x 10(9)
virus particles/liter. IFA and ELISA, using HHV-7/SupT1 cell lysate
as an antigen, seem to correlate well in detecting high and
low HHV-7 antibody in sera from chronic fatigue patients and
healthy donors as controls. HHV-7 from SupT1 cell culture was
free of HHV-6 and other human herpesviruses as tested by PCR,
and the HHV-7 PCR signal was still strong when the viral
preparation was diluted to 4.82 x 10(2) genome copies. Since
HCBMC are expensive to obtain and available in only small
amounts, it is difficult to obtain large quantities of HHV-7
antigen. On the other hand, the SupT1 cell is an excellent
source to produce consistently sufficient quantities of HHV-7
for purification studies, development of immunodiagnostics, in
vivo infectivity studies, evaluation of antiviral drugs, and
molecular biological studies.

Ablashi DV, Handy M, Bernbaum J, Chatlynne LG, Lapps W, Kramarsky B, 

Post a Comment

Featured Products From the ProHealth Store
Ultra EPA  - Fish Oil Energy NADH™ 12.5mg Mitochondria Ignite™ with NT Factor®

Looking for Vitamins, Herbs and Supplements?
Search the ProHealth Store for Hundreds of Natural Health Products

Article Comments

Be the first to comment on this article!

Post a Comment

Natural Pain Relief Supplements

Featured Products

Mitochondria Ignite™ with NT Factor® Mitochondria Ignite™ with NT Factor®
Reduce Fatigue up to 45%
Ultra EPA  - Fish Oil Ultra EPA - Fish Oil
Ultra concentrated source of essential fish oils
Energy NADH™ 12.5mg Energy NADH™ 12.5mg
Improve Energy & Cognitive Function
FibroSleep™ FibroSleep™
The All-in-One Natural Sleep Aid
Vitamin D3 Extreme™ Vitamin D3 Extreme™
50,000 IU Vitamin D3 - Prescription Strength

Natural Remedies

Three-Step Strategy to Reverse Mitochondrial Aging Three-Step Strategy to Reverse Mitochondrial Aging
"It's Not Easy Being Green" - But It Is Healthy
Block food Cravings At Their Molecular Root Block food Cravings At Their Molecular Root
Pioneer Scientists Uncover a Revolutionary Neuroprotective Supplement for Nerve Health Pioneer Scientists Uncover a Revolutionary Neuroprotective Supplement for Nerve Health
The Most Powerful Natural Antioxidant Discovered to Date - Hydroxytyrosol The Most Powerful Natural Antioxidant Discovered to Date - Hydroxytyrosol

ProHealth, Inc.
555 Maple Ave
Carpinteria, CA 93013
(800) 366-6056  |  Email

· Become a Wholesaler
· Vendor Inquiries
· Affiliate Program
Credit Card Processing
Be the first to know about new products, special discounts and the latest health news. *New subscribers only

CONNECT WITH US ProHealth on Facebook  ProHealth on Twitter  ProHealth on Pinterest  ProHealth on Google Plus

© 2016 ProHealth, Inc. All rights reserved. Pain Tracker App  |  Store  |  Customer Service  |  Guarantee  |  Privacy  |  Contact Us  |  Library  |  RSS  |  Site Map