ProHealth health Vitamin and Natural Supplement Store and Health
Home  |  Log In  |  My Account  |  View Cart  View Your ProHealth Vitamin and Supplement Shopping Cart
800-366-6056  |  Contact Us  |  Help
Facebook Google Plus
Fibromyalgia  Chronic Fatigue Syndrome & M.E.  Lyme Disease  Natural Wellness  Supplement News  Forums  Our Story
Store     Brands   |   A-Z Index   |   Best Sellers   |   New Products   |   Deals & Specials   |   Under $10   |   SmartSavings Club

Trending News

Is Coconut Oil Healthy? (The American Heart Association Doesn’t Think So)

American Heart Association Renders Itself Obsolete With 1960s Dietary Advice on Coconut Oil

How Fish Oil Benefits Heart Disease Patients with Depression

Ginger Fights Obesity

Health Benefits of Artichokes

Putting the Spotlight on Coriander Seed Oil

Migraines? Powdered Ginger May Help

Are Americans Really Getting Too Much Vitamin D? A Critical Look at Recent Media Warnings

Eating more vegetable protein may protect against early menopause

Vitamin C and antibiotics: A new one-two 'punch' for knocking-out cancer stem cells

 
Print Page
Email Article

Detection of Mycoplasma genus & Mycoplasma fermentans by PCR in patients with Chronic Fatigue Syndrome (CFS)

  [ 47 votes ]   [ Discuss This Article ]
By Vojdani A, Choppa PC, Tagle C, Andrin R, Samimi B, Lapp CW • www.ProHealth.com • December 15, 1998


Mycoplasma fermentans and other Mycoplasma species are
colonizers of human mucosal surfaces and may be associated
with human immunodeficiency virus infection. While many
infectious agents have been described in different percentages
of patients with Chronic Fatigue Syndrome (CFS), little is
known about the prevalence of mycoplasmas and especially M.
fermentans in CFS patients. A polymerase chain reaction
(PCR)-based assay was used to detect Mycoplasma genus and M.
fermentans genomes in peripheral blood mononuclear cells
(PBMC) of CFS patients. Blood was collected from 100 patients
with CFS and 50 control subjects. The amplified products of
717 bp of Mycoplasma genus, and 206 bp of M. fermentans were
detected in DNA purified from blood samples in 52% and 34% of
CFS samples, respectively. In contrast, these genomes were
found in only 14% and 8% of healthy control subjects
respectively (P < 0.0001). All samples were confirmed by
Southern blot with a specific probe based on internal
sequences of the expected amplification product. Several
samples, which were positive for Mycoplasma genus, were
negative for M. fermentans indicating that other Mycoplasma
species are involved. A quantitative PCR was developed to
determine the number of M. fermentans genome copies present in
1 microg of DNA for controls and CFS patients. Mycoplasma copy
numbers ranging from 130 to 880 and from 264 to 2400 were
detected in controls and CFS positive subjects, respectively.
An enzyme immunoassay was applied for the detection of
antibodies against p29 surface lipoprotein of M. fermentans to
determine the relationship between M. fermentans genome copy
numbers and antibody levels. Individuals with high genome copy
numbers exhibited higher IgG and IgM antibodies against M.
fermentans specific peptides. Isolation of this organism by
culture from clinical specimens is needed in order to
demonstrate specificity of signal detected by PCR in this
study.




Post a Comment

Featured Products From the ProHealth Store
Mitochondria Ignite™ with NT Factor® Ultra EPA  - Fish Oil Ultra ATP+, Double Strength

Looking for Vitamins, Herbs and Supplements?
Search the ProHealth Store for Hundreds of Natural Health Products


Article Comments



Be the first to comment on this article!

Post a Comment


 
NAD+ Ignite with Niagen

Featured Products

Ultra EPA  - Fish Oil Ultra EPA - Fish Oil
Ultra concentrated source of essential fish oils
FibroSleep™ FibroSleep™
The All-in-One Natural Sleep Aid
Ultra ATP+, Double Strength Ultra ATP+, Double Strength
Get energized with malic acid & magnesium
Energy NADH™ 12.5mg Energy NADH™ 12.5mg
Improve Energy & Cognitive Function
Optimized Curcumin Longvida® Optimized Curcumin Longvida®
Supports Cognition, Memory & Overall Health

Natural Remedies

Astaxanthin - A Little-Known but Power-Packed Nutrient Astaxanthin - A Little-Known but Power-Packed Nutrient
Coenzyme Q10 - The Energy Maker Coenzyme Q10 - The Energy Maker
The Revolutionary 'Good Fat' That Promotes Heart, Brain, Bone and Joint Health The Revolutionary 'Good Fat' That Promotes Heart, Brain, Bone and Joint Health
Can Glycine + Amino Acids Be the Secret to Deep, Rejuvenating Sleep? Can Glycine + Amino Acids Be the Secret to Deep, Rejuvenating Sleep?
Milk Thistle: Trusted Support for Health & Healing in a Toxic World Milk Thistle: Trusted Support for Health & Healing in a Toxic World

CONTACT US
ProHealth, Inc.
555 Maple Ave
Carpinteria, CA 93013
(800) 366-6056  |  Email

· Become a Wholesaler
· Vendor Inquiries
· Affiliate Program
SHOP WITH CONFIDENCE
Credit Card Processing
SUBSCRIBE TO OUR NEWSLETTERS
Get the latest news about Fibromyalgia, M.E/Chronic Fatigue Syndrome, Lyme Disease and Natural Wellness

CONNECT WITH US ProHealth on Facebook  ProHealth on Twitter  ProHealth on Pinterest  ProHealth on Google Plus

© 2017 ProHealth, Inc. All rights reserved. Pain Tracker App  |  Store  |  Customer Service  |  Guarantee  |  Privacy  |  Contact Us  |  Library  |  RSS  |  Site Map