ProHealth health Vitamin and Natural Supplement Store and Health
Home  |  Log In  |  My Account  |  View Cart  View Your ProHealth Vitamin and Supplement Shopping Cart
800-366-6056  |  Contact Us  |  Help
Facebook Google Plus
Fibromyalgia  Chronic Fatigue Syndrome & M.E.  Lyme Disease  Natural Wellness  Supplement News  Forums  Our Story
Store     Brands   |   A-Z Index   |   Best Sellers   |   New Products   |   Deals & Specials   |   Under $10   |   SmartSavings Club

Trending News

Is Magnesium the Missing Link in Your Heart Healthy Routine?

A Little Zinc Goes a Long Way

Supplementation with vitamin D associated with improved testosterone, erectile function among middle...

More evidence for calorie restriction’s longevity effect

Vitamin D deficiency increases risk of chronic headache

Wearable biosensors can flag illness, Lyme disease, risk for diabetes; low airplane oxygen

VIDEO: The Best Brain Foods That Help Increase Your Memory!

Iron (And More) For Lasting, Natural Energy

Affordable Care Act made cancer screening more accessible for millions, study finds

Metabolic syndrome increases the need for vitamin E

 
Print Page
Email Article

Elevated apoptotic cell population in patients with Chronic Fatigue Syndrome (CFS): the pivotal role of protein kinase RNA

  [ 21 votes ]   [ Discuss This Article ]
By Vojdani A, Ghoneum M, Choppa PC, Magtoto L, Lapp CW • www.ProHealth.com • December 15, 1997


OBJECTIVES: A prominent feature of chronic fatigue syndrome
(CFS) is a disordered immune system. Recent evidence indicates
that induction of apoptosis might be mediated in a
dysregulated immune system by the upregulation of growth
inhibitory cytokines. Therefore, the purpose of this study was
to evaluate the apoptotic cell population, interferon-alpha
(IFN-alpha) and the IFN-induced protein kinase RNA (PKR) gene
transcripts in peripheral blood lymphocytes (PBL) of CFS
individuals, as compared to healthy controls.

SUBJECTS AND
METHODS: PBL were isolated from CFS (n = 29) and healthy
control individuals (n = 15) and subjected to quantitative
analysis of apoptotic cell population and cell cycle
progression by flow cytometry. Quantitative competitive
polymerase chain reaction (Q/C PCR) and Western blot analysis
were used to assess the levels of PKR mRNA and protein in
control and CFS individuals. In addition, circulating
IFN-alpha was measured by ELISA assay.

RESULTS: Increased
apoptotic cell population was observed in CFS individuals, as
compared to healthy controls (26.6 +/- 12.9% and 9.9 +/- 4.2%,
respectively). The increased apoptotic subpopulation in CFS
individuals was accompanied by an abnormal cell arrest in the
S phase and the G2/M boundary of the cell cycle as compared to
the control group (8.6 +/- 1.2 to 22.8 +/- 2.4 and 3.6 +/-
0.82 to 24.3 +/- 3.4, respectively). In addition, CFS
individuals exhibited enhanced PKR mRNA and protein levels
(mean basal level 3538 +/- 1050 and 2.7 +/- 0.26,
respectively) as compared to healthy controls (mean basal
level 562 +/- 162 and 0.89 +/- 0.18, respectively). In 50% of
the CFS samples (n = 29) treated with 2-aminopurine (2-AP) (a
potent inhibitor of PKR) the apoptotic population was reduced
by more then 50%.

CONCLUSIONS: PKR-mediated apoptosis in CFS
individuals may contribute to the pathogenesis and the fatigue
symptomatology associated with CFS.




Post a Comment

Featured Products From the ProHealth Store
Vitamin D3 Extreme™ Ultra ATP+, Double Strength Energy NADH™ 12.5mg

Looking for Vitamins, Herbs and Supplements?
Search the ProHealth Store for Hundreds of Natural Health Products


Article Comments



Be the first to comment on this article!

Post a Comment


 
NAD+ Ignite with Niagen

Featured Products

Energy NADH™ 12.5mg Energy NADH™ 12.5mg
Improve Energy & Cognitive Function
Vitamin D3 Extreme™ Vitamin D3 Extreme™
50,000 IU Vitamin D3 - Prescription Strength
Mitochondria Ignite™ with NT Factor® Mitochondria Ignite™ with NT Factor®
Reduce Fatigue up to 45%
Ultra ATP+, Double Strength Ultra ATP+, Double Strength
Get energized with malic acid & magnesium
Ultra EPA  - Fish Oil Ultra EPA - Fish Oil
Ultra concentrated source of essential fish oils

Natural Remedies

Priming Your Immune System for Cold & Flu Season Priming Your Immune System for Cold & Flu Season
When a Negative is Positive - Goodnighties Recovery Sleepwear When a Negative is Positive - Goodnighties Recovery Sleepwear
Fighting Fatigue with Ground-breaking French Oak Wood Extract Fighting Fatigue with Ground-breaking French Oak Wood Extract
Physically and Mentally Exhausted? How to Restore Energy at Its Source Physically and Mentally Exhausted? How to Restore Energy at Its Source
The Crucial Role CoQ10 Plays in Fibromyalgia and ME/CFS The Crucial Role CoQ10 Plays in Fibromyalgia and ME/CFS

CONTACT US
ProHealth, Inc.
555 Maple Ave
Carpinteria, CA 93013
(800) 366-6056  |  Email

· Become a Wholesaler
· Vendor Inquiries
· Affiliate Program
SHOP WITH CONFIDENCE
Credit Card Processing
SUBSCRIBE TO OUR NEWSLETTERS
Get the latest news about Fibromyalgia, M.E/Chronic Fatigue Syndrome, Lyme Disease and Natural Wellness

CONNECT WITH US ProHealth on Facebook  ProHealth on Twitter  ProHealth on Pinterest  ProHealth on Google Plus

© 2017 ProHealth, Inc. All rights reserved. Pain Tracker App  |  Store  |  Customer Service  |  Guarantee  |  Privacy  |  Contact Us  |  Library  |  RSS  |  Site Map