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Development & application of HHV-6 antigen capture assay for the detection of HHV-6 infections

  [ 24 votes ]   [ Discuss This Article ]
www.ProHealth.com • September 15, 1996


An HHV-6 antigen capture assay measuring gp116/64/54 antigen was
developed. This ELISA is specific for HHV-6 Variants A and B,
does not cross react with other human herpesviruses, is
sensitive, stable, quantitative, and can detect antigen in
body fluids and cell cultures. Relative to virus isolation or
techniques for measuring HHV-6 nucleic acids, the assay is
much simpler and less expensive to perform. Plasmas/sera
(413) obtained from healthy donors, children with Exanthem
subitum, febrile illnesses, patients with Chronic Fatigue
Syndrome, and AIDS patients tested by antigen capture assay
demonstrated that the assay is useful in clinical laboratory
settings. The capture assay can also be used to monitor cell
cultures for virus isolation, production, quantitation, and
antiviral agent screening.

Marsh S, Kaplan M, Asano Y, Hoekzema D, Komaroff AL, Whitman JE Jr,
Ablashi DV




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