ProHealth health Vitamin and Natural Supplement Store and Health
Home  |  Log In  |  My Account  |  View Cart  View Your ProHealth Vitamin and Supplement Shopping Cart
800-366-6056  |  Contact Us  |  Help
Facebook Google Plus
Fibromyalgia  Chronic Fatigue Syndrome & M.E.  Lyme Disease  Natural Wellness  Supplement News  Forums  Our Story
Store     Brands   |   A-Z Index   |   Best Sellers   |   New Products   |   Deals & Specials   |   Under $10   |   SmartSavings Club

Trending News

Is a Good Night's Sleep at the Top of Your Wishlist?

Ashwagandha Helps Hormones - Aids Arthritis

Why You Should Be Eating More Porcini Mushrooms

A Breathalyzer for Disease?

How Bacopa Can Help Improve Your Cognitive Function

Magnesium Reduces Diabetes and Helps Keep You Young

Lavender Aromatherapy Can Ease Pre-Op Anxiety

Give Your Health a Much-Needed Boost With Geranium

The Role of Resveratrol in Achieving Optimal Health

Could Coconut Oil Help Reduce Antibiotics?

 
Print Page
Email Article

Detection of Il-1beta, Il-6 and Tnf-Alpha in Skin of Fibromyalgia Patients

  [ 69 votes ]   [ Discuss This Article ]
By Souzan Salemi, Janine Rethage, Lorenzo Kaeser, Uwe Wollina, Beat A. Michel, Renate E. Gay, Steffen Gay, Haiko Sprott • www.ProHealth.com • December 12, 2001





Fibromyalgia (FM) is a common, chronic musculoskeletal pain disorder of unknown etiology seen predominantly in women. As reported previously, highly ordered collagen cuffs were observed around preterminal nerve fibers in the skin of FM patients for unknown reasons. Therefore, the question was raised whether abnormal collagen metabolism is correlated with neurogenic inflammation, a potential activator of collagen metabolism, in FM patients. The presence of inflammatory cytokines, IL-1b, IL-6 and TNF-a was investigated by using reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC).

53 skin biopsies from FM patients (women, 30-65 years of age) were examined and compared to skin biopsies of 10 age- and gender-matched healthy controls. Biopsies were obtained from the deltoid region. One rheumatoid arthritis synovial tissue was used as a positive control for the expression of cytokines. Total RNA isolated from the tissue samples were reverse transcribed (RT) by random hexamers as the primer for RT followed by PCR amplification (40 cycles) using specific primers for IL-1b, IL-6 and TNF-a.

G3PDH was used as an internal control for loading differences. Negative controls included no template control and RNA control to check for genomic contamination. Expression of IL-1b and TNF-a protein was investigated in the skin by IHC using specific antibodies (avidin-biotin method). Rabbit polyclonal anti-human TNF-a antibodies and monoclonal anti-human IL-1b antibodies were employed as primary antibodies. Equal concentrations of purified rabbit and mouse IgG were used as negative control.

Positive signals (RT-PCR) were detected in 19/50 FM patients (38%) for IL-1b, in 14/51 FM patients (27%) for IL-6, and in 17/53 patients (32%) for TNF-a. None of the cytokines could be detected in healthy control skin. Immunoreactivity for IL-1b and TNF-a could be demonstrated in some skin tissues of FM patients. However, the number of positive cells and the intensity of staining was low.

In conclusion, the detection of cytokines in FM skin suggest the presence of inflammatory foci in the skin of these patients and may explain the response to NSAID therapy in a certain subset of FM patients.

Disclosure: Dr. Salemi was supported by the Zurzach foundation and Dr. Sprott by the American Fibromyalgia Syndrome Association (AFSA) and the Albert Böni foundation, all others by their institutions.



Post a Comment

Featured Products From the ProHealth Store
FibroSleep™ Ultra EPA  - Fish Oil Vitamin D3 Extreme™


Article Comments



Be the first to comment on this article!

Post a Comment


 
Optimized Curcumin Longvida with Omega-3

Featured Products

FibroSleep™ FibroSleep™
The All-in-One Natural Sleep Aid
Mitochondria Ignite™ with NT Factor® Mitochondria Ignite™ with NT Factor®
Reduce Fatigue up to 45%
Vitamin D3 Extreme™ Vitamin D3 Extreme™
50,000 IU Vitamin D3 - Prescription Strength
Ultra EPA  - Fish Oil Ultra EPA - Fish Oil
Ultra concentrated source of essential fish oils
Ultra ATP+, Double Strength Ultra ATP+, Double Strength
Get energized with malic acid & magnesium

Natural Remedies

Quercetin: Natural Support for Allergy & Inflammation Relief and More Quercetin: Natural Support for Allergy & Inflammation Relief and More
Strengthen Cell Function with Energy-Boosting Niagen Strengthen Cell Function with Energy-Boosting Niagen
IBS, Crohn’s Disease, Colitis, and Other Digestive Disorders IBS, Crohn’s Disease, Colitis, and Other Digestive Disorders
Research Links Green Tea to Weight Loss Research Links Green Tea to Weight Loss
Bone Broth Benefits for Digestion, Arthritis and Cellulite Bone Broth Benefits for Digestion, Arthritis and Cellulite

CONTACT US
ProHealth, Inc.
555 Maple Ave
Carpinteria, CA 93013
(800) 366-6056  |  Email

· Become a Wholesaler
· Vendor Inquiries
· Affiliate Program
SHOP WITH CONFIDENCE
Credit Card Processing
SUBSCRIBE TO OUR NEWSLETTERS
Get the latest news about Fibromyalgia, M.E/Chronic Fatigue Syndrome, Lyme Disease and Natural Wellness

CONNECT WITH US ProHealth on Facebook  ProHealth on Twitter  ProHealth on Pinterest  ProHealth on Google Plus

© 2017 ProHealth, Inc. All rights reserved. Pain Tracker App  |  Store  |  Customer Service  |  Guarantee  |  Privacy  |  Contact Us  |  Library  |  RSS  |  Site Map