ProHealth health Vitamin and Natural Supplement Store and Health
Home  |  Log In  |  My Account  |  View Cart  View Your ProHealth Vitamin and Supplement Shopping Cart
800-366-6056  |  Contact Us  |  Help
Facebook Google Plus
Fibromyalgia  Chronic Fatigue Syndrome & M.E.  Lyme Disease  Natural Wellness  Supplement News  Forums  Our Story
Store     Brands   |   A-Z Index   |   Best Sellers   |   New Products   |   Deals & Specials   |   Under $10   |   SmartSavings Club

Trending News

Fighting Heartburn and Gerd Naturally – And Safely!

Natural Bladder Control, Go Less and Live More

Vital Molecule Increases Cellular Energy and Improves Cognitive Function

Top Vitamin and Mineral Deficiencies — Are You at Risk?

Trimming the spare tire: Canola oil may cut belly fat

How Pomegranate May Protect Against Cancer

Omega Fix for Obesity: How the Right Fats Fight Fat

The Onion: Cancer Fighter and Food Preserver

Probiotics improve cognition in Alzheimer's patients

Curcumin Reverses the Cellular Damage of Chronic Stress

 
Print Page
Email Article

Opioid Receptor Positive Cells in Fibromyalgia Muscle Tissue

  [ 30 votes ]   [ Discuss This Article ]
www.ProHealth.com • January 21, 2002




Souzan Salemi, Lorenzo Kaeser, Janine Rethage, Beat A. Michel, Renate E. Gay, Steffen Gay, Haiko Sprott, Zurich, Switzerland

Dysregulation of kappa (KOR) and delta opioid (DOR) receptors may be a reason for severe and persistent pain and therapeutical failure in fibromyalgia (FM) patients. The aim of the present study was to localize these receptors in muscle tissues by in-situ RT-PCR and immunohistochemical (IHC) methods.

Muscle biopsies were obtained from 12 patients diagnosed for FM according to the ACR criteria (all women, 30-65 years of age). Normal brain tissue was taken as a positive control. Pieces of muscle samples from each patient were rapidly frozen in isopentane, cooled with liquid nitrogen and covered with OCT (Tissue-Tek®) embedding medium. 6 cryostat sections were fixed and used for further experiments. To search for the cellular localization of the opioid receptors mRNA in the tissues in-situ RT-PCR on muscle tissues and normal brain tissue was performed. Rabbit polyclonal IgG anti-human KOR antibodies (60711A, Pharmingen, San Diego, CA), and rabbit polyclonal IgG anti-human DOR antibodies (DiaSorin, Minnesota) were employed as primary antibodies for IHC. To examine whether opioid receptors are produced in satellite cells, monoclonal antibodies against human CD34 (Pharmingen) a marker of quiescent satellite cells in muscle, were used for double labeling.

DOR mRNA was abundantly expressed in normal brain as well as in the muscle of FM patients detected by in-situ RT-PCR. DOR mRNA was observed on the surface of certain muscle fibers. Immunoreactivity for KOR or DOR was seen in muscle tissues of normal individuals and FM patients. In FM muscle, DOR was distributed more widely than KOR which is in accordance with our previous results showing higher expression of DOR by real-time PCR. These receptors were also found to be expressed on the surface of certain muscle cells. By utilizing antibodies against human CD34 a similar pattern of expression could be observed suggesting the expression of opioid receptors in satellite cells.

Since satellite cells contribute to repair and regeneration of damaged myofibers. The present study for the expression of opioid receptors in muscle satellite cells may help in understanding pathophysiology and pain in FM patients.

Disclosure: Dr. Salemi was supported by the Zurzach foundation and Dr. Sprott by the American Fibromyalgia Syndrome Association and all others by their institutions.



Post a Comment

Featured Products From the ProHealth Store
Ultra ATP+, Double Strength Optimized Curcumin Longvida® FibroSleep™


Article Comments



Be the first to comment on this article!

Post a Comment


 
Natural Pain Relief Supplements

Featured Products

Ultra ATP+, Double Strength Ultra ATP+, Double Strength
Get energized with malic acid & magnesium
Energy NADH™ 12.5mg Energy NADH™ 12.5mg
Improve Energy & Cognitive Function
Mitochondria Ignite™ with NT Factor® Mitochondria Ignite™ with NT Factor®
Reduce Fatigue up to 45%
Ultra EPA  - Fish Oil Ultra EPA - Fish Oil
Ultra concentrated source of essential fish oils
Vitamin D3 Extreme™ Vitamin D3 Extreme™
50,000 IU Vitamin D3 - Prescription Strength

Natural Remedies

Vitamin K-2 – A Key Player in Cardiovascular and Bone Health Vitamin K-2 – A Key Player in Cardiovascular and Bone Health
Cell-Charging Compound Gives Steady Energy to Fibromyalgia & Chronic Fatigue Patients Cell-Charging Compound Gives Steady Energy to Fibromyalgia & Chronic Fatigue Patients
Three-Step Strategy to Reverse Mitochondrial Aging Three-Step Strategy to Reverse Mitochondrial Aging
Red Yeast Rice - Natural Option for Supporting Healthy Cholesterol Red Yeast Rice - Natural Option for Supporting Healthy Cholesterol
Reversing Neurodegeneration with a New Magnesium Compound Reversing Neurodegeneration with a New Magnesium Compound

CONTACT US
ProHealth, Inc.
555 Maple Ave
Carpinteria, CA 93013
(800) 366-6056  |  Email

· Become a Wholesaler
· Vendor Inquiries
· Affiliate Program
SHOP WITH CONFIDENCE
Credit Card Processing
SUBSCRIBE TO OUR NEWSLETTERS
Get the latest news about Fibromyalgia, M.E/Chronic Fatigue Syndrome, Lyme Disease and Natural Wellness

CONNECT WITH US ProHealth on Facebook  ProHealth on Twitter  ProHealth on Pinterest  ProHealth on Google Plus

© 2016 ProHealth, Inc. All rights reserved. Pain Tracker App  |  Store  |  Customer Service  |  Guarantee  |  Privacy  |  Contact Us  |  Library  |  RSS  |  Site Map