ProHealth health Vitamin and Natural Supplement Store and Health
Home  |  Log In  |  My Account  |  View Cart  View Your ProHealth Vitamin and Supplement Shopping Cart
800-366-6056  |  Contact Us  |  Help
Facebook Google Plus
Fibromyalgia  Chronic Fatigue Syndrome & M.E.  Lyme Disease  Natural Wellness  Supplement News  Forums  Our Story
Store     Brands   |   A-Z Index   |   Best Sellers   |   New Products   |   Deals & Specials   |   Under $10   |   SmartSavings Club

Trending News

Scientifically-designed fasting diet lowers risks for major diseases

More evidence for calorie restriction’s longevity effect

Supplementation with vitamin D associated with improved testosterone, erectile function among middle...

Vitamin D deficiency increases risk of chronic headache

Acupuncture boosts effectiveness of standard medical care for chronic pain, depression

Get the Most From Your Green Tea

VIDEO: The Best Brain Foods That Help Increase Your Memory!

Iron (And More) For Lasting, Natural Energy

Metabolic syndrome increases the need for vitamin E

Study provides new evidence that exercise is not key to weight control

Print Page
Email Article

Opioid Receptor Positive Cells in Fibromyalgia Muscle Tissue

  [ 30 votes ]   [ Discuss This Article ] • January 21, 2002

Souzan Salemi, Lorenzo Kaeser, Janine Rethage, Beat A. Michel, Renate E. Gay, Steffen Gay, Haiko Sprott, Zurich, Switzerland

Dysregulation of kappa (KOR) and delta opioid (DOR) receptors may be a reason for severe and persistent pain and therapeutical failure in fibromyalgia (FM) patients. The aim of the present study was to localize these receptors in muscle tissues by in-situ RT-PCR and immunohistochemical (IHC) methods.

Muscle biopsies were obtained from 12 patients diagnosed for FM according to the ACR criteria (all women, 30-65 years of age). Normal brain tissue was taken as a positive control. Pieces of muscle samples from each patient were rapidly frozen in isopentane, cooled with liquid nitrogen and covered with OCT (Tissue-Tek®) embedding medium. 6 cryostat sections were fixed and used for further experiments. To search for the cellular localization of the opioid receptors mRNA in the tissues in-situ RT-PCR on muscle tissues and normal brain tissue was performed. Rabbit polyclonal IgG anti-human KOR antibodies (60711A, Pharmingen, San Diego, CA), and rabbit polyclonal IgG anti-human DOR antibodies (DiaSorin, Minnesota) were employed as primary antibodies for IHC. To examine whether opioid receptors are produced in satellite cells, monoclonal antibodies against human CD34 (Pharmingen) a marker of quiescent satellite cells in muscle, were used for double labeling.

DOR mRNA was abundantly expressed in normal brain as well as in the muscle of FM patients detected by in-situ RT-PCR. DOR mRNA was observed on the surface of certain muscle fibers. Immunoreactivity for KOR or DOR was seen in muscle tissues of normal individuals and FM patients. In FM muscle, DOR was distributed more widely than KOR which is in accordance with our previous results showing higher expression of DOR by real-time PCR. These receptors were also found to be expressed on the surface of certain muscle cells. By utilizing antibodies against human CD34 a similar pattern of expression could be observed suggesting the expression of opioid receptors in satellite cells.

Since satellite cells contribute to repair and regeneration of damaged myofibers. The present study for the expression of opioid receptors in muscle satellite cells may help in understanding pathophysiology and pain in FM patients.

Disclosure: Dr. Salemi was supported by the Zurzach foundation and Dr. Sprott by the American Fibromyalgia Syndrome Association and all others by their institutions.

Post a Comment

Featured Products From the ProHealth Store
Ultra EPA  - Fish Oil Energy NADH™ 12.5mg Vitamin D3 Extreme™

Looking for Vitamins, Herbs and Supplements?
Search the ProHealth Store for Hundreds of Natural Health Products

Article Comments

Be the first to comment on this article!

Post a Comment

NAD+ Ignite with Niagen

Featured Products

Ultra EPA  - Fish Oil Ultra EPA - Fish Oil
Ultra concentrated source of essential fish oils
Energy NADH™ 12.5mg Energy NADH™ 12.5mg
Improve Energy & Cognitive Function
Mitochondria Ignite™ with NT Factor® Mitochondria Ignite™ with NT Factor®
Reduce Fatigue up to 45%
FibroSleep™ FibroSleep™
The All-in-One Natural Sleep Aid
Ultra ATP+, Double Strength Ultra ATP+, Double Strength
Get energized with malic acid & magnesium

Natural Remedies

Strontium - The Missing Mineral for Strong Bones Strontium - The Missing Mineral for Strong Bones
Dreaming of a Good Night's Sleep? Dreaming of a Good Night's Sleep?
Coenzyme Q10 - The Energy Maker Coenzyme Q10 - The Energy Maker
Restoring Gut Health: How to Create a Firewall Against Toxins Entering the Gut Wall Restoring Gut Health: How to Create a Firewall Against Toxins Entering the Gut Wall
Secret Nutrient for Radiant Skin Secret Nutrient for Radiant Skin

ProHealth, Inc.
555 Maple Ave
Carpinteria, CA 93013
(800) 366-6056  |  Email

· Become a Wholesaler
· Vendor Inquiries
· Affiliate Program
Credit Card Processing
Get the latest news about Fibromyalgia, M.E/Chronic Fatigue Syndrome, Lyme Disease and Natural Wellness

CONNECT WITH US ProHealth on Facebook  ProHealth on Twitter  ProHealth on Pinterest  ProHealth on Google Plus

© 2017 ProHealth, Inc. All rights reserved. Pain Tracker App  |  Store  |  Customer Service  |  Guarantee  |  Privacy  |  Contact Us  |  Library  |  RSS  |  Site Map