ProHealth health Vitamin and Natural Supplement Store and Health
Home  |  Log In  |  My Account  |  View Cart  View Your ProHealth Vitamin and Supplement Shopping Cart
800-366-6056  |  Contact Us  |  Help
Facebook Google Plus
Fibromyalgia  Chronic Fatigue Syndrome & M.E.  Lyme Disease  Natural Wellness  Supplement News  Forums  Our Story
Store     Brands   |   A-Z Index   |   Best Sellers   |   New Products   |   Deals & Specials   |   Under $10   |   SmartSavings Club

Trending News

Astaxanthin Is a Longevity Promoter

Meta-analysis links vitamin D deficiency with increased risk of mortality during 10.5 year median pe...

Prevent Or Reverse Diabetes: An Amazing Herbal Intervention

Exercise and Vitamin D Better Together for Heart Health

VIDEO: Essential Oils for Weight Loss

Can Valerian Root Help You Sleep Better?

Eight servings of veggies a day is clearly best for the heart

Can Ginkgo Give Your Brain a Boost?

Fighting Statin-Induced Diabetes with CoQ10

The Many Wonders of Calming Sandalwood Oil

 
Print Page
Email Article

Abstract: Ribonuclease L proteolysis in peripheral blood mononuclear cells of chronic fatigue syndrome patients

  [ 10 votes ]   [ Discuss This Article ]
www.ProHealth.com • July 16, 2002




Source: J Biol Chem 2002 Jul 12; [epub ahead of print]

Demettre E, Bastide L, D'Haese A, De Smet K, De Meirleir K, Tiev KP, Englebienne P, Lebleu B.

UMR 5124, CNRS, Montpellier 34293.

A 37 kDa binding polypeptide accumulates in peripheral blood mononuclear cells (PBMC) extracts from Chronic Fatigue Syndrome (CFS) patients and is being considered as a potential diagnostic marker (1). We establish here that this low molecular weight 2-5A binding polypeptide is a truncated form of the native 2-5A dependent ribonuclease L (RNase L), generated by an increased proteolytic activity in CFS PBMC extracts. RNase L proteolysis in CFS PBMC extracts can be mimicked in a model system in which recombinant RNase L is treated with human leukocyte elastase (HLE). RNase L proteolysis leads to the accumulation of two major fragments with molecular weights of 37 and 30 kDa.

The 37 kDa fragment includes the 2-5A binding site and the N-terminal end of native RNase L. The 30 kDa fragment includes the catalytic site in the C-terminal part of RNase L. Interestingly, RNase L remains active and 2-5A dependent when degraded into its 30 kDa and 37 kDa fragments by proteases of CFS PBMC extract or by purified HLE. The 2-5A dependent nuclease activity of the truncated RNase L could result from the association of these digestion products, as suggested in pull down experiments. (1)De Meirleir, K., Bisbal, C., Campine, I., De Becker, P., Salehzada, T., Demettre, E., and Lebleu, B. (2000) Am J Med 108(2), 99-105.

PMID: 12118002 [PubMed - as supplied by publisher]




Post a Comment

Featured Products From the ProHealth Store
Vitamin D3 Extreme™ Ultra EPA  - Fish Oil Optimized Curcumin Longvida®

Looking for Vitamins, Herbs and Supplements?
Search the ProHealth Store for Hundreds of Natural Health Products


Article Comments



Be the first to comment on this article!

Post a Comment


 
NAD+ Ignite with Niagen

Featured Products

Mitochondria Ignite™ with NT Factor® Mitochondria Ignite™ with NT Factor®
Reduce Fatigue up to 45%
Vitamin D3 Extreme™ Vitamin D3 Extreme™
50,000 IU Vitamin D3 - Prescription Strength
Energy NADH™ 12.5mg Energy NADH™ 12.5mg
Improve Energy & Cognitive Function
Optimized Curcumin Longvida® Optimized Curcumin Longvida®
Supports Cognition, Memory & Overall Health
Ultra EPA  - Fish Oil Ultra EPA - Fish Oil
Ultra concentrated source of essential fish oils

Natural Remedies

Mitochondria-Booster NIAGEN® Shows Promise in First Human Clinical Trial Mitochondria-Booster NIAGEN® Shows Promise in First Human Clinical Trial
Safely Burn Away Body Fat Safely Burn Away Body Fat
Could a B-12 Deficiency Be Causing Your Symptoms? Could a B-12 Deficiency Be Causing Your Symptoms?
Pioneer Scientists Uncover a Revolutionary Neuroprotective Supplement for Nerve Health Pioneer Scientists Uncover a Revolutionary Neuroprotective Supplement for Nerve Health
Guarding Against the Dangers of Vitamin D Deficiency Guarding Against the Dangers of Vitamin D Deficiency

CONTACT US
ProHealth, Inc.
555 Maple Ave
Carpinteria, CA 93013
(800) 366-6056  |  Email

· Become a Wholesaler
· Vendor Inquiries
· Affiliate Program
SHOP WITH CONFIDENCE
Credit Card Processing
SUBSCRIBE TO OUR NEWSLETTERS
Get the latest news about Fibromyalgia, M.E/Chronic Fatigue Syndrome, Lyme Disease and Natural Wellness

CONNECT WITH US ProHealth on Facebook  ProHealth on Twitter  ProHealth on Pinterest  ProHealth on Google Plus

© 2017 ProHealth, Inc. All rights reserved. Pain Tracker App  |  Store  |  Customer Service  |  Guarantee  |  Privacy  |  Contact Us  |  Library  |  RSS  |  Site Map