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In Vitro Study of the Efficacy of ImmunePro RX and Foscarnet in Eliminating the Infectivity of HHV-6A

  [ 39 votes ]   [ Discuss This Article ]
www.ProHealth.com • September 25, 2002


Mitchell Abrahams(1), Louise G. Chatlynne(2), Roger Mazlen(1), and Dharmam V. Ablashi(2)

(1)BioMolecular Sciences, Inc., Marina Del Rey, CA, (2)Advanced Bio Technologies Inc., Columbia, MD.

HHV-6A is the most common strain of the virus found in cases of multiple sclerosis (MS) and Chronic Fatigue Syndrome (CFS). Thus far Foscarnet is the drug that has been found to be the most effective against HHV-6A infection, but physicians are reluctant to use it because of its toxicity.

Here we demonstrate the ImmunePro Rx, biologically active whey protein, is effective against HHV-6 either alone or in combination with Foscarnet. Cultures of HSB-2 cells, a T-lymphoblastoid cell line derived from a patient with acute lymphoblastic leukemia, were infected with HHV-6A, GS Strain at an multiplicity of infection (MOI) that caused 42% of the cells to die in 10 days (assay 1). These cultures were then treated with ImmunePro Rx (IP) at 1,000, 100, 1, 0.1, or 0 µg/mL. Uninfected cells were also dosed at the same concentration to test for cytotoxicity. The same test system was used to test combinations of ImmunePro Rx and Foscarnet.

In the assay a higher HHV-6A MOI was used so that only 12% of the cells were left alive after 7 days (assay 2). In this assay, Foscarnet was tested at 300, 200, 30, 15, and 7.5 µg/mL IP. A cytotoxicity control without virus was run for both assays. After assay 1 was cultured for 10 days and assay 2 was cultured for 7 days, a cytoproliferation assay was run on the cultures. The cytoproliferation assay uses a fluorescent dye (Alamar Blue) that measures reduction/oxidation.

The values for replicate cultures at the same experimental condition were averaged. The negative control uninfected cells with no experimental reagent, was fixed at 100%, and all other experimental conditions were calculated as a percentage of the negative control. IP demonstrated virtually no toxicity at any concentration used. When used alone Foscarnet demonstrated severe toxicity at 300 µg/mL and mild toxicity at all other concentrations used. When used in combination with IP at 1,000 µg/mL, Foscarnet had only mild toxicity at 300 and 200 µg/mL, and none at lower concentrations. With 125 µg/mL IP the cytotoxicity of Foscarnet was also reduced, but not as dramatically.

In assay 1 (IP alone, lower viral MOI) with 1,000 µg/mL of ImmunePro Rx, no viral activity was evident (99.5% of negative control), at 100 µg/mL partial inhibition of the virus was noted (79.7% of negative control).

In assay 2 (drug combination, higher viral MOI), neither IP nor Foscarnet alone was efficient at maintaining healthy cells in the presence of virus, but in combination both concentrations of IP were able to eliminate all (100% with 1,000 µg/mL IP) or nearly all (94.3% of negative control with 125 µg/mL IP) the HHV-6 with only 7.5 µg/mL of Foscarnet present. At levels of Foscarnet above 30 µg/mL the efficacy of the combinations fell off due to the cytotoxicity of the Foscarnet. IP is able to eliminate lower levels of HHV-6 infectivity by itself, and in combination with low levels of Foscarnet it can eliminate very high levels of HHV-6. IP can counter the cytotoxic effects of Foscarnet.

Source: BioMolecular Sciences, Inc.



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