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A mutagenic PCR identifies isolates of Borrelia garinii responsible for Lyme borreliosis.

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Abstract

Borrelia garinii is one of the three major Borreliae responsible for
Lyme borreliosis in Europe. We have characterized a protein of B. garinii (VS102) and a genomic fragment from the gene encoding this protein was cloned. The DNA sequence of the fragment showed high homology with a known gene of B. burgdorferi sensu stricto. The protein encoded by this gene in B. burgdorferi sensu stricto is a phosphocarrier protein (histidine-containing protein). A mutation T to G polymorphism at codon 57 was found to be specific to B. garinii. A PCR-based approach that allows the rapid detection of this mutation made it possible to specifically discriminate B. garinii from other B. burgdorferi genospecies with high sensitivity and specificity.

FEMS Microbiol Lett. 2000 Oct 15;191(2):199-203. Evaluation Studies; Research Support, Non-U.S. Gov’t

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