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A streptavidin-biotin-based microarray platform for immunoassays.

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Abstract

The authors describe a microarray system for
disease diagnosis based on antibody-antigen interactions. Either biotinylated antibodies or antigens are coupled via streptavidin linkers onto a gold surface. This platform has been used to establish recombinant antibody-antigen interactions and to detect specific IgM antibodies in sera of patients suspected of
Lyme borreliosis. Therefore, this microarray system can be adapted for further applications.

Methods Mol Biol. 2004;264:73-83. Research Support, Non-U.S. Gov’t

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