Abstract: Noninvasive diagnosis of arthritis by autofluorescence

Invest Radiol. 2003 Sep;38(9):578-83.

Hansch A, Sauner D, Hilger I, Frey O, Haas M, Malich A, Brauer R, Kaiser WA.

Institute of Diagnostic and Interventional Radiology, Friedrich Schiller University-Jena, Bachstrasse 18, 07740 Jena, Germany. andreas.hansch@med.uni-jena.de

RATIONALE AND OBJECTIVES: The detection of arthritis by autofluorescence was investigated using an antigen-induced arthritis model.

METHODS: For autofluorescence investigations of joints, a mobile fluorescence-detector was constructed consisting of a lens/mirror system attached to a conventional spectrofluorometer and optimized fiber optic cables reaching to and from the site of investigation. Autofluorescence measurements were performed at 7 arthritic and 7 healthy mice. Fifteen antigen-induced arthritis and 3 healthy mice were used for histologic examinations.

RESULTS: In the exudative stage (day 1), a decrease of emission signal intensities for excitation wavelengths at 300 nm (emission, 355-365 nm) and 360 nm (emission, 475-485 nm) was observed. Signals increased on day 7 (maximum of cellular infiltration). Chronic inflammation (day 14 and 21) led to a decrease of signals again.

CONCLUSION: Arthritis influences autofluorescence signals in vivo. The detected excitation/emission pairs can be assigned to collagen/elastin and NAD(P)H. Signal intensities of NAD(P)H differed significantly from controls at day 1 and 7.

PMID: 12960527 [PubMed – in process]

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