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An indirect microhemagglutination test for Lyme disease.

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Abstract

We have developed an indirect microhemagglutination test (IMHA) for detecting antibodies to Borrelia burgdorferi using formalin-fixed chicken erythrocytes sensitized with a sonicated extract of the bacteria. The specificity of IMHA for
Lyme disease was compared with that of a conventional indirect immunoperoxidase technique (IP) using sera from the patients with syphilis, randomly-chosen outpatients unrelated to
Lyme disease, and from normal individuals. The IMHA was more specific than IP, giving little cross-reaction with syphilis. The IMHA also gave little background reactions with the sera of individuals unrelated to
Lyme disease or syphilis. Therefore, the IMHA could be a useful test for the serodiagnosis of
Lyme disease.

Microbiol Immunol. 1991;35(7):589-92. Comparative Study

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