Autoantibodies to nuclear envelope antigens in Chronic Fatigue Syndrome (CFS)

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We have identified and partially characterized the

autoantibodies in sera of 60 patients with chronic fatigue

syndrome. Approximately 52% of the sera were found to react

with nuclear envelope antigens. The combination of nuclear rim

staining observed in immunofluorescence microscopy and

immunoblot analysis of highly purified nuclear envelope

proteins provided initial characterization of these

autoantibodies. Further characterization showed that some

sera immunoprecipitated the in vitro transcription and

translation product of a human cDNA clone encoding the

nuclear envelope protein lamin B1. The autoantibodies were of

the IgG isotype. The occurrence of autoantibodies to a

conserved intracellular protein like lamin B1 provides new

laboratory evidence for an autoimmune component in chronic

fatigue syndrome.

MCM: Used 1988 CDC criteria. Control groups were (1) 10 w

Major depression, 10 with allergies, and 10 in good health, &

(2) 41 healthy blood donors. Of the CFS pts, 50% had psych

disorders, 50% FM, 28% positive Schirmer tests, and 58%

concurrent allergies. 68% were ANA positive, 52% had nuclear

rim staining, 47% had nucleoplasmic and nucleolar staining

(one colocalizing with p80 coilin) [to be separately

reported], and 33% had thread-like wavy cytoplasmic staining

in a vimentin-like pattern.

Konstantinov K, von Mikecz A, Buchwald D, Jones J, Gerace L, Tan EM