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Human babesiosis is caused predominantly by B. microti and B. divergens, a protozooan parasites of red blood cells. Both are transmitted by Ixodes ricinus ticks, also the primary vector of
Lyme disease. Clinical manifestation varied widely from asymptomatic infection to a serve rapidly fatal
disease. The diagnosis of babesiosis include examination of stained blood smers, serological evaluation indirect antibody tests and PCR. With the evolution PCR–based techniques, the diagnosis and monitoring of babesial infections became more sensitive and reliable.