Editor’s comment: Ocimum basilicum is the scientific name for basil. Basil leaves produce essential oils that are rich in monoterpenes, which are known to have antihyperalgesic properties. (They counteract an increased sensitivity to pain.) Cyclodextrins are a family of compounds made up of sugar molecules bound together in a ring. They are sometimes used in the manufacture of foods and pharmaceutical drugs to make them more water-soluble. In this study, essential oils from basil leaves were combined with cyclodextrins to make them more usable by the body, then tested on mice with fibromyalgia. The researchers found that with or without the cyclodextrins, the basil leaf essential oils had a significant analgesic effect on chronic pain.
Note: You may download the full text of this article free HERE.
Cyclodextrin-Complexed Ocimum basilicum Leaves Essential Oil Increases Fos Protein Expression in the Central Nervous System and Produce an Antihyperalgesic Effect in Animal Models for Fibromyalgia.
Subscribe to the World's Most Popular Newsletter (it's free!)
O. basilicum leaves produce essential oils (LEO) rich in monoterpenes. The short half-life and water insolubility are limitations for LEO medical uses. ?-Cyclodextrin (?-CD) has been employed to improve the pharmacological properties of LEO. We assessed the antihyperalgesic profile of LEO, isolated or complexed in ?-CD (LEO/?-CD), on an animal model for fibromyalgia.
Behavioral tests: mice were treated every day with either LEO/?-CD (25, 50 or 100 mg/kg, p.o.), LEO (25 mg/kg, p.o.), tramadol (TRM 4 mg/kg, i.p.) or vehicle (saline), and 60 min after treatment behavioral parameters were assessed. Therefore, mice were evaluated for mechanical hyperalgesia (von Frey), motor coordination (Rota-rod) and muscle strength (Grip Strength Metter) in a mice fibromyalgia model. After 27 days, we evaluated the central nervous system (CNS) pathways involved in the effect induced by experimental drugs through immunofluorescence protocol to Fos protein.
The differential scanning analysis (DSC), thermogravimetry/derivate thermogravimetry (TG/DTG) and infrared absorption spectroscopy (FTIR) curves indicated that the products prepared were able to incorporate the LEO efficiently. Oral treatment with LEO or LEO-?CD, at all doses tested, produced a significant reduction of mechanical hyperalgesia and we were able to significantly increase Fos protein expression.
Together, our results provide evidence that LEO, isolated or complexed with ?-CD, produces analgesic effects on chronic non-inflammatory pain as fibromyalgia.
Source: International Journal of Molecular Science, December 29, 2014. By Simone S. Nascimento, Adriano A. S. Araújo, Renan G. Brito, Mairim R. Serafini, Paula P. Menezes, Josimari M. DeSantana, Waldecy Lucca Júnior, Pericles B. Alves, Arie F. Blank, Rita C. M. Oliveira, Aldeidia P. Oliveira, Ricardo L. C. Albuquerque-Júnior, Jackson R. G. S. Almeida and Lucindo J. Quintans-Júnior. Laboratory of Pre-Clinical Pharmacology (LAPEC), Department of Physiology, Federal University of Sergipe, Av. Tancredo Neves, S/N, Rosa Elza, CEP: 49.000-100, São Cristóvão, Sergipe 49.100-000, Brazil.