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Characterization of Borrelia burgdorferi strains in Lyme arthritis.

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Abstract

In the study presented, we investigated whether
Lyme arthritis is associated with a particular Borrelia burgdorferi genospecies. Using the PCR technique, in 7/11 samples of synovial fluid of patients with
Lyme arthritis a part of the ospA-gene was identified and the strains characterized by sequencing of the amplified DNA. Borrelia burgdorferi sensu stricto was found in 3 patients, B. garinii in 3, and B. afzelii in 1 patient. In conclusion,
Lyme arthritis is caused by all 3 human pathogenetic genospecies which are actually known. For clinical practice PCR proved to be a rather insensitive diagnostic method, but may confirm the diagnosis of
Lyme arthritis in doubtful cases.

Scand J Infect Dis. 1998;30(3):265-8. Research Support, Non-U.S. Gov’t

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