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To evaluate the diagnostic value of the polymerase chain reaction (PCR) to detect Borrelia burgdorferi DNA in patients with ocular
Of 256 consecutive uveitis patients six selected individuals with clinical evidence for
Lyme borreliosis and 30 patients with non-
Lyme uveitis were enrolled.
Lyme serology was performed by ELISA and western blotting. Urine samples were examined by an optimised nested polymerase chain reaction (PCR) protocol.
Only four of six uveitis patients suspected for
Lyme borreliosis were ELISA positive, while all six subjects showed a positive western blot. B burgdorferi PCR was positive in all of these six patients. Whereas two of the 30 controls had a positive
Lyme serology, B burgdorferi DNA was not detectable by PCR in any sample from these patients.
PCR for the detection of B burgdorferi DNA in urine of uveitis patients is a valuable tool to support the diagnosis of ocular
Lyme borreliosis. Moreover, these patients often show a weak humoral immune response which may more sensitively be detected by immunoblotting.