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Detection of heterogeneity of Borrelia burgdorferi in ixodes ticks by culture-dependent and culture-independent methods.

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Abstract

Abilities to detect heterogeneity of ospC genotypes of the
Lyme disease spirochete Borrelia burgdorferi in the tick vector by in vitro culture (IVC) and direct PCR (dPCR) were compared. IVC failed to detect one-third of the ospC genotypes detected by dPCR. Among IVC results, common ospC genotypes were overrepresented while occurrence of rare genotypes was underestimated.

J Clin Microbiol. 2013 Feb;51(2):615-7. doi: 10.1128/JCM.03009-12. Epub 2012 Nov 21. Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov’t

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