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The aim of our work was to determine the sero-prevalence of antibodies to B. burgdorferi in 300 patients living in Calabria, a region of southern Italy. The research of antibodies to B. burgdorferi was conducted by ELISA as a screening method and Western Blot as a confirmatory test. Out of the 300 patients we found a rate of positivity of 7.3% with ELISA and 4.5% with Western Blot. This data allows us to classify Calabria, among the regions where
Lyme borreliosis is non-endemic and where sero-epidemiological research has been carried out on patients, as having a degree of positivity second to that of Campania (9.1%) and higher than Lombardy (3.2%) and Umbria (2.8%). Considering all the demographic and clinical data, only that of the higher IgG positivity of males over females is statistically significant.