The diagnosis of
Lyme disease is very complicated and a single diagnostic method cannot exclude infection. We assessed the performance of two commercially available Borrelia burgdorferi rapid diagnostic tests (RDT) in comparison to multiple laboratory-based diagnostic assays using specimens with a gradually increasing probability of Borrelia infection. Based on 200 specimens, the analytical sensitivities for IgG and IgM were 18 and 23 % for the
Lyme RDT and 24 and 32 % for the Borreliose Complete RDT. The sensitivity for detecting diagnosed
Lyme borreliosis cases was low (26 %
Lyme RDT and 32 % with the Borreliose Complete RDT respectively), whereas the specificity was good (85 %
Lyme RDT and 88 % Borreliose Complete). Based on this evaluation, the performance of RDTs in detecting
Lyme borreliosis appeared to be below that of laboratory-based diagnostics.