Evidence Fibromyalgia Is an Immune Problem

Evidence Fibromyalgia Is an Immune ProblemThis study demonstrated that fibromyalgia patients have unique immunologic traits – evidence that fibromyalgia may actually have an immunologic basis rather than rheumatologic or neurologic as once thought. 

The researchers also developed a novel test protocol, which they suggest may be useful as a tool for diagnosing fibromyalgia. 

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Unique immunologic patterns in fibromyalgia
– Source: BMC Clinical Pathology, December 17, 2012

By Frederick G. Behm, et al.


Background: Fibromyalgia (FM) is a clinical syndrome characterized by chronic pain and allodynia. The diagnosis of FM has been one of exclusion as a test to confirm the diagnosis is lacking.

Recent data highlight the role of the immune system in FM. Aberrant expressions of immune mediators, such as cytokines, have been linked to the pathogenesis and traits of FM. We therefore determined whether cytokine production by immune cells is altered in FM patients by comparing the cellular responses to mitogenic activators of stimulated blood mononuclear cells of a large number of patients with FM to those of healthy matched individuals.

Methods: Plasma and peripheral blood mononuclear cells (PBMC) were collected from 110 patients with the clinical diagnosis of FM and 91 healthy donors. Parallel samples of PBMC were cultured overnight in medium alone or in the presence of mitogenic activators; PHA or PMA in combination with ionomycin. The cytokine concentrations of IFN-y, IL-5, IL-6, IL-8, IL-10, MIP-1? , MCP-1, and MIP1-? in plasma as well as in cultured supernatants were determined using a multiplex immunoassay using bead array technology.

Results: Cytokine levels of stimulated PBMC cultures of healthy control subjects were significantly increased as compared to matched non-stimulated PBMC cultures.

In contrast, the concentrations of most cytokines were lower in stimulated samples from patients with FM compared to controls. The decreases of cytokine concentrations in patients samples ranged from 1.5-fold for MIP-1? to 10.2-fold for IL-6 in PHA challenges.

In PMA challenges, we observed 1.8 to 4-fold decreases in the concentrations of cytokines in patient samples.

Conclusion: The cytokine responses to mitogenic activators of PBMC isolated from patients with FM were significantly lower than those of healthy individuals, implying that cell-mediated immunity is impaired in FM patients.

This novel cytokine assay reveals unique and valuable immunologic traits, which, when combined with clinical patterns, can offer a diagnostic methodology in FM.

BMC Clinical Pathology, December 17, 2012. By Frederick G Behm1, Igor M Gavin2, Oleksiy Karpenko2, Valerie Lindgren1, Sujata Gaitonde1, Peter A Gashkoff1 and Bruce S Gillis1. 1 Department of Pathology, University of Illinois at Chicago (UIC), Chicago, IL, USA. 2 Research Resource Center, University of Illinois at Chicago, Chicago, IL, USA. E-mail: bsgmd@hotmail.com

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