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Identification of Borrelia burgdorferi ribosomal protein L25 by the phage surface display method and evaluation of the protein’s value for serodiagnosis.

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Abstract

The phage surface display technique was used to identify Borrelia burgdorferi antigens. By affinity selection with immunoglobulin G from pooled sera of six
Lyme borreliosis (LB) patients, the ribosomal protein L25 was identified. The diagnostic value of L25 was investigated by an enzyme-linked immunosorbent assay, using sera from 80 LB patients and 75 controls, and the use of the protein resulted in a specificity of 99% and a 23% sensitivity, which qualify L25 as a useful antigen when combined with others.

J Clin Microbiol. 2006 Oct;44(10):3778-80. Research Support, Non-U.S. Gov’t

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