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To determine variability, if exists, in interleukin-6 (IL-6) response by different cell cultures stimulated by Borrelia burgdorferi.
Five human embryonic diploid fibroblast cell lines (three of them of lung origin) and four human synovial cell cultures were used. Induction of IL-6 was made with B. burgdorferi B31 culture and E. coli lipopolysaccharide (LPS). IL-6 was determined by a commercial EIA (Coaliza IL-6).
All cell cultures produced IL-6 under basal conditions and responded after adding E. coli LPS. The IL-6 production after B. burgdorferi stimulation was higher than that induced by LPS with three out the four synovial cell cultures and lowest with lung fibroblasts.
The ability of B. burgdorferi to stimulate IL-6 production depends on the nature and origin of cell cultures. This variability and the fact that the most reactive cell cultures were those of synovial precedence may correlated with some clinical aspects of the