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Interaction of Lyme disease spirochetes with cultured eucaryotic cells.

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Abstract

The association of the
Lyme disease spirochete, Borrelia burgdorferi, with cultured human endothelial cells was investigated. Attachment was time and temperature dependent, with optimal adherence occurring after 4 h of incubation at 37 degrees C. Pretreatment of borreliae with heat, immune human serum, or monoclonal antibodies directed against outer surface protein B (OspB) reduced the attachment of organisms to host cell monolayers. These results suggest that the adherence of B. burgdorferi may be mediated, at least in part, by borrelial surface proteins.

Infect Immun. 1989 Apr;57(4):1324-6. Research Support, U.S. Gov’t, P.H.S.

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