In this article – which you can read in its entirety at www.landesbioscience.com/journals/40/article/12486/ (click on "Download PDF") – the researchers provide further detail regarding the multiple detection methods that enabled them “to observe XMRV infection in our CFS cohort.” Directing the information to other researchers, they recommend use of more than one type of assay in future studies of XMRV as it may relate to human disease. Note that Table 4 lists the results of each test used for each of the 101 patients/samples they tested (cDNA nested PCR, DNA nested PCR, LNCaP co-culture with PMCs, Antibody in plasma, LNCaP culture with plasma).
Following is the article's summary/abstract:
(Mikovits, et al. Virulence, Sep-Oct 2010)
In October 2009, we reported the first direct isolation of infectious xenotropic murine leukemia virus-related virus (XMRV). In that study, we used a combination of biological amplification and molecular enhancement techniques to detect XMRV in more than 75% of 101 patients with chronic fatigue syndrome (CFS). Since our report, controversy arose after the publication of several studies that failed to detect XMRV infection in their CFS patient populations.
In this addenda, we further detail the multiple detection methods we used in order to observe XMRV infection in our CFS cohort.
• Our results indicate that PCR from DNA of unstimulated peripheral blood mononuclear cells is the least sensitive method for detection of XMRV in subjects' blood.
• We advocate the use of more than one type of assay in order to determine the frequency of XMRV infection in patient cohorts in future studies of the relevance of XMRV to human disease.