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Molecular cloning and DNA sequencing of OspC gene of two strains Borrelia burgdorferi isolated in China.

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To investigate the variation of OspC gene in two Chinese isolates of Borrelia burgdorferi.


PCR technique was used to amplify the OspC gene from the whole cellular DNA of isolates BT01 and BJ-9011. The amplified products were inserted into plasmid pGEM-3ZF(+) and sequenced.


Except the signal peptide, the OspC genes of the two isolates BT01 and BJ-9011 were 579 bp and 576 bp which encode 193, 192 amino acids respectively. The nucleotide and amino acids sequence identity between the two strains was 86% and 83%. High homology exists between these Chinese isolates and several foreign isolates (PBi, PKo, B31), especially in BJ-9011. It had 99% nucleotide and amino acid sequence identified with B31.


Variations in OspC genes are noted between the two Chinese Borrelia burgdorferi isolates and foreign isolates.

Zhonghua Yi Xue Za Zhi. 1998 Jul;78(7):551-3. English Abstract

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