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Molecular cloning and DNA sequencing of OspC gene of two strains Borrelia burgdorferi isolated in China.

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Abstract

OBJECTIVE:

To investigate the variation of OspC gene in two Chinese isolates of Borrelia burgdorferi.

METHODS:

PCR technique was used to amplify the OspC gene from the whole cellular DNA of isolates BT01 and BJ-9011. The amplified products were inserted into plasmid pGEM-3ZF(+) and sequenced.

RESULTS:

Except the signal peptide, the OspC genes of the two isolates BT01 and BJ-9011 were 579 bp and 576 bp which encode 193, 192 amino acids respectively. The nucleotide and amino acids sequence identity between the two strains was 86% and 83%. High homology exists between these Chinese isolates and several foreign isolates (PBi, PKo, B31), especially in BJ-9011. It had 99% nucleotide and amino acid sequence identified with B31.

CONCLUSION:

Variations in OspC genes are noted between the two Chinese Borrelia burgdorferi isolates and foreign isolates.

Zhonghua Yi Xue Za Zhi. 1998 Jul;78(7):551-3. English Abstract

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