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Phylogeographic analysis reveals a complex population structure of Borrelia burgdorferi in southeastern and south central Canada.

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Abstract

Lyme disease, caused by the bacterium Borrelia burgdorferi sensu stricto, is an emerging zoonotic
disease in Canada and is vectored by the blacklegged tick Ixodes scapularis. Here we used Bayesian analyses of Sequence Types (STs), determined by Multi-Locus Sequence Typing (MLST), to investigate the phylogeography of B. burgdorferi populations in southern Canada and the USA by analysing MLST data from 564 B. burgdorferi-positive samples collected in surveillance. A total of 107 Canadian samples from field sites were characterised as part of this study, and these data were combined with existing MLST data for samples from the USA and Canada. Only 17% of STs were common between both countries while 49% occurred only in the USA, and 34% occurred only in Canada. However, STs in southeastern Ontario and southwestern Quebec were typically identical to those in northeastern USA, suggesting a recent introduction into this region from the USA. In contrast, STs in other locations in Canada (the Maritimes; Long Point, Ontario; and southeastern Manitoba) were frequently unique to those locations, but were putative descendants of STs previously recorded in the USA. The picture in Canada is consistent with relatively recent introductions from multiple refugial populations in the USA. These data thus point to a geographic pattern of populations of B. burgdorferi in North America that may be more complex than simply comprising Northeast, Midwest and Californian groups. We speculate that this reflects the complex ecology and spatial distribution of key reservoir hosts.

Copyright © 2014, American Society for Microbiology. All Rights Reserved.

Appl Environ Microbiol. 2014 Dec 12. pii: AEM.03730-14. [Epub ahead of print]

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