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Search for the spirochete Borrelia burgdorferi sensu lato by polymerase chain reaction in wild Chilean ticks.

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Lyme disease is a tick-borne human
disease caused by the spirochete Borrelia burgdorferi. Main vectors of
Lyme disease are ticks of the Ixodes and Amblyomma genera. Cases with clinical manifestations of
Lyme disease and favorable responses to antimicrobial agents have been reported in Chile, some of them with erythema migrans, the hallmark of B burgdorferi infection.


To detect the presence of B burgdorferi in Chilean ticks.


A total of 62 ticks were recollected from wild rodents and cervidae in the Southern region of Chile. Infected and non infected ticks of the species Ixodes ricimus, were used as controls. Insects were homogenised and B burgdorferi was detected using classical and nested polymerase chain reactions.


B burgdorferi was not detected in the studied ticks.


Although all the elements required for the enzootic cycle of B burgdorferi are present in Chile, its direct detection in Chilean ticks using the nested polymerase chain reaction assay was negative.

Rev Med Chil. 2001 Mar;129(3):270-6. English Abstract; Research Support, Non-U.S. Gov’t

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