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The 83-kilodalton antigen of Borrelia burgdorferi which stimulates immunoglobulin M (IgM) and IgG responses in infected hosts is expressed by a chromosomal gene.

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Abstract

We report the cloning and characterization of a chromosomal gene from Borrelia burgdorferi expressing an 83-kilodalton protein antigen in Escherichia coli. The antigen reacted strongly with antisera from two human
Lyme disease patients. The chromosomal gene was expressed in a 6.5-kilobase-pair ClaI fragment cloned into a variable-reading-frame plasmid vector.

J Clin Microbiol. 1990 Jul;28(7):1673-5. Research Support, Non-U.S. Gov’t; Research Support, U.S. Gov’t, P.H.S.

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