By means of indirect fluorescent antibody assay (IFA), Borrelia burgdorferi B31 strain was used for antigen, to detect the 2579 serum specimens from forest area in 9 counties of Fujian province, 47 cases in 8 counties were detected positive, positive rate was 1.82%. By epidemiological investigation, we found that peoples who infected with
Lyme disease, 91.67% of them (22/24) have been bited by Ixodes, and 6 cases suffer typical clinical symptoms of
Lyme disease, 9 cases have the similar or somewhat typical symptoms. We found
Lyme disease could be cross-reaction to leptospirosis, in this paper the serum specimens which
Lyme disease and leptospirosis all positive, we were not consider them to
Lyme disease for time being, no matter the patients may be have suffer double infection. The indentification of the cross-reaction between
Lyme disease and leptospirosis should be further studied.