The Effect of d-Lenolate® on the Immune Parameters in Humans

Source – The Science Behind d-Lanelate® Studies 1999 – 2014. East Park™ Research

Background and Introduction

The purpose of this study was to test the immune effects of d-Lenolate in healthy
volunteers at the Department of Cytogenetics and Immunology of NICS. d-Lenolate is a
dietary supplement patented by East Park Research. d-Lenolate formulation is prepared
on a patented extraction process of selected olive leaves that contain Oleuropein. This
study will examine the effect of 21 days of d-Lenolate treatment on the immune
parameters of healthy volunteers.

Immune-toxicology examines the damaging/modifying effects caused by
exposure at the workplace, environment or therapy on the immune system. Its task is to
detect and assess the modifying factors affecting the immune system especially from the
aspect of their effect on human health. An immune response may be elicited when the
immune system is the passive target of a chemical agent or when the chemical, as
antigen, triggers a specific response. In consequence of the complexity of the immune
system the chemical agents have a broad target of attack. They can affect the
development, maturation, division, differentiation and function of cells, or modify the
regulation of the immune system.

The immunology tests were carried out on White blood cells (WBC). WBC is
involved in all aspects of the immune reaction and is an important role in the defense
mechanisms of the body. WBC can be broken down into 3 main types: lymphocytes,
monocytes and granulocytes. Monocytes make up about 2-9% of WBC and are activated
by lymphokines that are secreted. As a result they become able to phagocytose foreign
matter such as bacteria, and can release a number of inflammatory mediators.

Lymphocytes become ‘activated’ when they encounter the foreign object/molecule for
which they are designed. We can respond to at least two million different foreign
molecules because our lymphocytes are pre-programmed to recognize them over the
course of our lifetimes, but only when they encounter those specific molecules.

Granulocytes has a very important role in the development if inflammatory and allergic
reactions. Most of the granulocytes are made up of neutrophils. Neutrophils are the basis
of cellular protection against infection, and can enter the tissues in large quantities. In the
course of bacterial or fungal infection, the neutrophil granulocytes, phagocytose and
destroy the pathogens. Oxygen-independent enzymes and oxygen-dependent enzymatic
systems achieve this intracellular killing of pathogens.

The activated phagocytic cells produce antimicrobial relative radicals, so called Reactive
Oxygen Intermediates (ROI). ROI is the ability of the neutrophils described above to kill
foreign cells or other unrecognized materials in the blood or tissues. The ROI molecules
are very toxic and therefore kept within the neutrophils and only exposed to the target
once it is engulfed by the neutrophil. Therefore, the killing capacity of WBC was
determined by measuring the production of ROI.

Methods

30 healthy volunteers, 15 men and 15 women, participated in a 21 day d-Lenolate
treatment which consisted of taking 2 capsules 3 times a day. The measurements were
done on the 1st, 8th, 15th, and 21st day. Blood samples were taken for determining the
qualitative and quantitative blood count for the immunology tests and were analyzed by
the lab.

Results and Discussion

In this study, we see that a greater number of lymphocytes are ‘activated’ as seen by the
development of certain surface markers. It is not clear why these cells are ‘activated’.
One idea is that they were sluggish before and now recognizes the foreign molecules they
were supposed to recognize before. However, the ROI production of neutrophils
increased significantly in both the control and the stimulated samples from the first week
of the treatment with continual growth throughout the study.

The neutrophils responded to several foreign molecules that were presented to them in blood samples. A (weak) stimulus, fMLP chemotatic peptide, was able to stimulate increased ROI response. A
particulate (solid) stimulus using, E. coli coated with antibodies, also increased the ROI
response, and PMA (a strong signal) increased the amount of ROI. The slight increase in
WBC is entirely due to an increase in the number of neutrophils.

In conclusion d-Lenolate showed a potential immune building response along with the ability to fight off
weak, solid, and strong bacterial stimuli.

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