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Hydrogel biomarker capturing microparticles were evaluated as a biomaterial to amplify the sensitivity of urine testing for infectious
Lyme disease is a bacterial infection transmitted by ticks. Early diagnosis and prompt treatment of
Lyme disease reduces complications including arthritis and cardiac involvement. While a urine test is highly desirable for
Lyme disease screening, this has been difficult to accomplish because the antigen is present at extremely low concentrations, below the detection limit of clinical immunoassays. N-isopropylacrylamide (NIPAm)-acrylic acid (AAc) microparticles were covalently functionalized with amine containing dyes via amidation of carboxylic groups present in the microparticles. The dyes act as affinity baits towards protein analytes in solution. NIPAm/AAc microparticles functionalized with acid black 48 (AB48) mixed with human urine, achieved close to one hundred percent capture and 100 percent extraction yield of the target antigen. In urine, microparticles sequestered and concentrated
Lyme disease antigens 100 fold, compared to the absence of microparticles, achieving an immunoassay detection sensitivity of 700 pg/mL in 10 mL urine. Antigen present in a single infected tick could be readily detected following microparticle sequestration. Hydrogel microparticles functionalized with high affinity baits can dramatically increase the sensitivity of urinary antigen tests for infectious diseases such as
Lyme disease. These findings justify controlled clinical studies evaluating the sensitivity and precision of
Lyme antigen testing in urine.
Copyright © 2010 Elsevier Ltd. All rights reserved.
Biomaterials. 2011 Feb;32(4):1157-66. doi: 10.1016/j.biomaterials.2010.10.004. Epub 2010 Oct 28. Evaluation Studies; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov’t; Research Support, U.S. Gov’t, Non-P.H.S.