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We studied the prevalence of anti-Borrelia burgdorferi antibodies in a sample of 1100 Greek male Navy recruits. Thirty-six (3.27%) subjects were found IgG positive by an enzyme immunoassay. Only three of the positive subjects (0.27%) were reactive by an IgG Western Blot. The seroprevalence of
Lyme disease is very low in the Greek young male adult population.