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Antibodies against Borrelia duttoni using indirect immunofluorescence could be demonstrated in 6 patients with erythema chronicum migrans and in 8 persons with tick-borne meningopolyneuritis. Significant increases of IgG and IgM antibody titres in the course of the
disease and IgG antibodies in the CSF indicate recent contact with Borrelia duttoni or a closely related agent. Demonstration by fluorescence serology of spirochaetaceae in Ixodes ricinus in two sites of infection equally indicate such an aetiology. The immunofluorescence test for patient sera used here improves the diagnosis of erythema chronicum migrans infection and of its various organ manifestations. Results are similar to those in
Lyme disease in the United States.