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Lyme alert: New Borrelia culture test to confirm active infection & diagnosis, track therapy success

  [ 31 votes ]   [ 1 Comment ] • September 14, 2011

[Note: Lyme authority Joseph Burrascano, MD, reportedly served as a consultant in the development and confirmation of this still 'investigational' test, and suggests it could revolutionize Lyme detection and treatment.]

Sharon Hill PA, Sep 5, 2011: Lyme Disease blood testing has been notorious for its unreliability This has been responsible for misdiagnoses and inappropriate patient care, as well as confusion on the part of both patient and physician alike.

Now, as a result of intensive research, Advanced Laboratory Services Inc. is able to offer what will rapidly become the new gold standard of Lyme tests, the Borrelia Culture.

Until now, the most widely used confirmatory tests for Lyme disease have been serologies.

• Being indirect tests, at best they can only indicate possible exposure to this organism at some previous point in time. It has been variously reported that the sensitivity of these assays is low and may miss anywhere from 30% to as many as 70% of cases of Lyme.

• Once positive, these serologic tests tend to remain positive for variable periods, even years, even after treatment.

• Therefore they do not and cannot be used as a marker for progress during treatment or for success of treatment. In addition, some acute viral infections may potentially give a false positive result.

How are most other infectious diseases diagnosed?

Traditionally, a culture is taken to see if an infection is present, and if so, what specific bacterium is causing it. Unfortunately, because Lyme Borrelia are symbionts, meaning that they need a living host to survive, trying to get them to thrive in vitro has been a nearly impossible task.

In addition, they are noted for their very slow growth.

Because of these difficulties, Borrelia culture until now has not been available to clinicians.

Advanced Laboratory Services Inc. is proud to announce that they have overcome many of these technical difficulties and is able to offer Borrelia cultures.

While still considered investigational, the new methods employed allow these advantages:

• By definition, culture is a direct test and if positive, indicates that an infection was present at the time the specimen was taken

• Cultures may be positive even in a patient who is seronegative

• In theory, any fluid or tissue that is infected can be cultured

• All known strains of Borrelia burgdorferi sensu lato can be detected

• When combined with PCR and DNA sequencing, the exact identity of the Borrelia can be ascertained

• Advanced methods have the promise to increase yield and decrease turn-around time

• Culture positivity fulfills even the strict CDC surveillance case definition

• Will become the new Gold Standard for laboratory testing

Currently, Advanced Labs is offering two Borrelia blood culture panels.

• The Basic Panel consists of culturing, with confirmation of identity by histology and by specific immunostaining. The report, if positive, will include a picture of the actual immunostained culture result. The expected turnaround time for this is approximately 10 to 14 days. However, some do grow more slowly, so all cultures will be held in the lab for a minimum of six weeks.

• We also offer the Extended Panel. In addition to histology and immunostaining, the identity of all positive cultures will be further studied by polymerase chain reaction (PCR) and by nucleic acid sequencing. These extra steps will generally require an additional 10 to 14 days. However, if the initial histology with immunostaining is positive, a preliminary report will be issued while the nucleic acid studies are pending.

Borrelia burgdorferi Culture Nuts and Bolts

• Sensitivity exceeds 80% if the patient is symptomatic at the time of blood draw and not on antibiotics for at least four weeks

• All negative controls have remained negative so far

• As with all blood cultures, may need several sets to be sure

• Any positive test is significant, even if it is the only positive out of a set of three

We have found that the success of culturing Borrelia can be increased by following these simple recommendations:

• The patient should not have been exposed to any antibiotics, even those not known to affect this organism, for a minimum of four weeks prior to the blood sample being drawn.

Borrelia are more likely to be recovered from patients who are symptomatic at the time of blood sampling.

• A higher yield may be seen if the blood is drawn in the early afternoon, when most infected patients feel especially ill.

To Contact Advanced Laboratory Services



Source: Advanced Laboratory Services, Sep 5, 2011

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Article Comments Post a Comment

Posted by: bettyg
Sep 21, 2011
WHOOPIE! I'm anxious to see the potential of this in helping us getting more accurate reports on blood lab testings. BettyG, Iowa lyme activist
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